安吉白茶白化叶和返绿叶叶绿体超微结构和叶绿素及其前体物含量的比较  

Comparison of Ultrastructure, Chlorophyll and Precursor Contents of Chloroplasts between Albino Leaves and Green Leaves in Camellia sinensis ‘Baiyel 1’

作　　者：杨荧 吕立堂 赵德刚 YANG Ying;LV Li-tang;ZHAO De-gang(College of Tea/Research Institute of Agricultural Bioengineering,Guizhou University,key Laboratory of Germplasm Innovation for Conservation and Protection of Mountain Plant Resources,Guiyang,Guizhou 550025,China;Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006,China)

机构地区：[1]贵州大学生命科学学院/农业生物工程研究院,山地植物资源保护与保护种质创新教育部重点实验室,贵州贵阳550025 [2]贵州大学茶学院/农业生物工程研究院,山地植物资源保护与保护种质创新教育部重点实验室,贵州贵阳550025 [3]贵州省农业科学院,贵州贵阳550006

出　　处：《山地农业生物学报》2019年第6期71-75,79,共6页Journal of Mountain Agriculture and Biology

基　　金：国家自然科学基金(31160149);贵州茶产业技术创新中心(黔科中引地[2017]4005)

摘　　要：安吉白茶(Camellia sinensis.'Baiye1')是典型的白化茶树品种,为了对其叶片白化现象进行探究,本研究观察了安吉白茶的白化叶和返绿叶的叶绿体超微结构,发现白化叶叶绿体内部结构发育不良,基粒片层排列疏松散乱,片层间缝隙大,层次不清晰,甚至断裂不成形,根据对叶绿素及其前体物质含量的测定和对叶绿素合成的相关基因在两个阶段叶片中的表达量测定结果发现,Mg-proto Ⅸ在白化叶中含量要高于返绿叶,推测叶绿素合成阶段的Mg-proto Ⅸ合成Pchlide a时受阻,确定安吉白茶发生白化是由于叶绿体发育不良,同时叶绿素合成阶段的Mg-proto Ⅸ合成Pchlide a时受阻所致。Camellia sinensis cv.Baiye1 is a typical albino tea cultivar.In order to investigate the albinism phenomenon of its leaves,the chloroplast ultrastructure of the albino leaves and the green leaves of Camellia sinensis cv.Baiye1 were observed,and it was found that the internal structure of the chloroplasts in the albino leaves was stunted.The grana lamellae were scattered and loosely arranged with big gaps,and the lamellar structure was not clear,even fractured.According to the determination of the content of chlorophyll and its precursors,and the expression of genes related to chlorophyll synthesis in leaves of two stages,it was found that the content of Mg-proto Ⅸ in albino leaves was significantly higher than that in returning green leaves and speculated that the synthesis of Pchlide a from Mg-proto Ⅸ was blocked in the chlorophyll synthesis pathway.These results indicate that the albinism of Camellia sinensis cv.Baiye1 was due to chloroplast dysplasiaand the blocking synthesis of Pchlide a from Mg-proto Ⅸ.

关 键 词：安吉白茶 叶绿体 超微结构 叶绿素前体物 

分 类 号：S571.1[农业科学—茶叶生产加工]