采后番木瓜果实炭疽病病原菌分离鉴定及其拮抗菌的筛选  

Isolation and Identification of Anthracnose Pathogen in Postharvest Papaya Fruits and Screening of the Antagonistic Bacteria

作　　者：王雅君 邵远志[1] 何文琪 尤文静 葛春晖 李雯[2] WANG Ya-jun;SHAO Yuan-zhi;HE Wen-qi;YOU Wen-jing;GE Chun-hui;LI Wen(College of Food Science and Engineering,Hainan University,Haikou 570228,China;College of Horticulture Science,Hainan University,Haikou 570228,China)

机构地区：[1]海南大学食品科学与工程学院,海南海口570228 [2]海南大学园艺学院,海南海口570228

出　　处：《食品工业科技》2020年第6期111-118,130,共9页Science and Technology of Food Industry

基　　金：国家重点研发计划(2016YFD0400904)

摘　　要：从采后发病番木瓜果实上分离炭疽病的病原菌,同时采用平板对峙法和滤纸片法从番木瓜果园土壤中筛选得到炭疽菌的拮抗菌W-2。通过形态学和分子生物学分别鉴定了病原菌和拮抗菌相关种属,并测试了拮抗菌W-2的拮抗活性。结果表明:番木瓜炭疽病病原菌鉴定为番木瓜炭疽菌Glomerella magna,拮抗菌W-2为萎缩芽孢杆菌(Bacillus atrophaeus)。拮抗菌W-2能够引起Glomerella magna菌丝体膨大和孢子畸形,且W-2浓度为1×108CFU/m L时,对菌丝生长抑制作用最强,浓度为1×10~8和1×10~9CFU/m L时,对孢子萌发的抑制作用最为显著(P<0.05)。活体生防实验表明,浓度为1×108CFU/m L的W-2菌悬液处理能够有效控制番木瓜果实炭疽病病斑的扩展,并显著降低果实的发病率(P<0.05)。The anthracnose pathogen was isolated from the infected papaya fruits,the antagonistic bacteria W-2 was screened from the soil in papaya orchard by the method of flat-stand method and the filter paper method. Both the pathogen and antagonistic bacteria were identified by morphological and molecular biological methods.The antagonistic activities of W-2 were studied preliminarily.Results showed that the pathogen was identified as Glomerella magna and the antagonistic bacteria was Bacillus atrophaeus.Enlarged mycelia and deformed spores were observed by the action of W-2.Moreover,the mycelial growth were inhibited at the concentration of 1 × 10~8 CFU/m L,and spore germination were inhibited significantly( P < 0.05) at the concentration of 1 × 10~8 and 1 × 10~9 CFU/m L.The in vivo test showed that the lesion diameter in papaya fruits was suppressed by W-2 at the concentration of 1 × 10~8 CFU/m L,and the disease incidence of fruits was reduced significantly( P < 0.05).

关 键 词：番木瓜果实 炭疽病 病原菌 拮抗细菌 分离鉴定 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]