代谢组及其在真菌研究中的应用  

Metabolomics and its use in fungal metabolic analyses: a review

作　　者：刘宏有 陈柳龙 高江涛 LIU Hong-You;CHEN Liu-Long;GAO Jiang-Tao(College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学生命科学学院

出　　处：《菌物学报》2019年第12期2078-2086,共9页Mycosystema

摘　　要：代谢组学是利用现代分析化学手段对一定条件下生物体内小分子代谢产物(初级和次级代谢产物)定性及定量,从而揭示生命现象及其内在规律的学科。相对于基因组、转录组和蛋白质组,代谢组是一定条件下生物学过程完成后的最终代谢产物的集合,因而是各种组学研究中最接近表型的一种组学,可以直接动态地反映出细胞的生理生化过程,从而有效地检测和发现特定的生化途径,准确地解释生理或者病理现象。代谢组学作为系统生物学中基因组学、转录组学以及蛋白质组学三大组学的延伸和补充,是目前的研究热点之一。目前代谢组学在真菌领域的研究得到日益重视和发展。本文首先从历史发展和技术路线简述了代谢组学的发展历程和常见的代谢组学研究方法。接着从真菌的分类鉴定、生物膜研究、代谢途径、代谢工程、天然产物发现与植物互作这6个方面介绍了代谢组学在真菌研究领域的应用。Metabolomics is a discipline that uses modern analytical chemistry to characterize and quantify small molecule metabolites(primary and secondary metabolites)in a given environment,thereby revealing life phenomena and their inherent laws.Relative to the genome,transcriptome and proteome,the metabolome is a collection of final metabolites after completion of biological processes under certain conditions,and thus is the closest phenotype of omics in various omics studies,and can directly and dynamically reflects the physiological and biochemical processes of the cells,so that specific biochemical pathways can be effectively detected and discovered,thereby accurately explaining physiological or pathological phenomena.Metabolomics is one of the current research hotspots as an extension and complement of the three major omics of genomics,transcriptomics and proteomics in system biology.At present,the research of metabolomics in the field of fungi has been extensively developed.This paper briefly describes the development of metabolomics in fungal research in seven aspects:fungal classification,biofilm research,metabolic pathway,metabolic engineering,natural product discovery and fungi-plant interaction.

关 键 词：代谢组 质谱 核磁共振 

分 类 号：Q949.32[生物学—植物学]