比较分析草菇与其他真菌直系同源基因的关系  

Genome-wide analysis of the orthologous genes between Volvariella volvacea and other selected fungi

作　　者：陶永新[1,2] 车金超 宋寒冰 李自燕 段静怡 Irum Mukhtar 谢宝贵[2] TAO Yong-Xin;CHE Jin-Chao;SONG Han-Bing;LI Zi-Yan;DUAN Jing-Yi;Irum Mukhtar;XIE Bao-Gui(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Mycological Research Center,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Ocean College,Minjiang University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]福建农林大学菌物研究中心,福建福州350002 [3]闽江学院海洋学院,福建福州350002

出　　处：《菌物学报》2019年第12期2144-2160,共17页Mycosystema

基　　金：福建省自然科学基金(2019J01380);福建农林大学园艺学院青年学术骨干培养基金(61201400706);福建农林大学科技创新专项基金(CXZX2017184);国家重点基础研究发展计划项目“食用菌产量和品质形成的分子机理及调控”(2014CB138302);木生型食用菌品种选育与产业化工程项目(fjzycxny2017010);福建省食用菌产业体系站长(K83139296);国家自然科学基金(31801920)~~

摘　　要：担子菌类的食用菌种类多、价值高、产量大,然而其产业的升级发展需要对食用菌生长发育相关生物学问题进行深入解析。目前多种食用菌完成了全基因组测序,然而作为非模式种其与模式丝状真菌间的直系同源基因目前尚缺乏全基因组水平的系统研究,在一定程度上限制了其分子生物学研究的深入。本研究以草菇为参照物种,将其与几种食用菌和模式丝状真菌进行两两直系同源基因分析,并对多物种间不同类型的直系同源基因进行功能富集。结果显示:一对一直系同源基因较多富集于基因复制、转录、翻译、修饰、加工等保守的基本功能类别;非一对一直系同源基因多属于基因家族,且包含了65%的转录因子,功能上富集在碳水化合物、脂质、氨基酸、次生代谢物及外源物质的代谢通路。无直系同源基因则较多富集在与基因重组、修复、信号转导相关的功能类别、特导性转录因子以及未知的预测基因。结果为食用菌分子生物学的深入研究提供有价值的参考。Basidiomycete edible fungi have high value and large yield. The upgrade and development of edible fungal industry requires in-depth analysis of biological issues involved in growth and development of edible fungi. At present, several edible fungi have completed the whole genome sequencing;however, the lack of systematic genome-wide research of the orthologous genes between non-model edible fungi and model filamentous fungi limits in-depth study of their molecular biology to some extent. In this study, straw mushroom Volvariella volvacea as the reference species was used to analyze the orthologous genes of straw mushroom and other several edible fungi and model filamentous fungi, and the functional enrichment of different types of orthologous genes was performed multispecifically. The results showed that the one-to-one orthologous genes were more enriched in the basic and conserved functional categories including gene replication, transcription, translation, modification and processing. The non one-to-one orthologous genes mainly belonged to gene family, and also included over 65% of the whole transcription factors, being enriched in the transport and metabolic pathways of carbohydrates, lipids, amino acids, secondary metabolites and exogenous substances. Non orthologous genes were enriched in the functional categories involved in gene recombination, repair, signal transduction, specific transcription factors and predicted genes with unknown functions. The results provide valuable information for further in-depth study of molecular biology in edible fungi.

关 键 词：食用真菌 丝状真菌 基因组 直系同源基因 

分 类 号：S646.13[农业科学—蔬菜学]