下丘脑弓状核PYY对大鼠摄食、胃运动和能量代谢的影响及机制  

Effects of Hypothalamic ARC PYY on the Feeding, Hastric Motility and Energy Metabolism in Rats and its Potential Mechanism

作　　者：薛秀菊 聂文波 冷慧[1] 王茜 孙向荣[1] 郭菲菲[1] 徐珞[1] XUE Xiu-ju;NIE Wen-bo;LENG Hui;WANG Qian;SUN Xiang-rong;GUO Fei-fei;XU Luo(Qingdao University,Qingdao,Shandong,266021,China;Heze City Single County Central Hospital,Heze,Shandong,274000,China;Heze City Single County Central Hospital,Department of orthopaedics,Heze,Shandong,274000,China)

机构地区：[1]青岛大学,山东青岛266021 [2]菏泽市单县中心医院,山东菏泽274000 [3]菏泽市单县中心医院骨科,山东菏泽274000

出　　处：《现代生物医学进展》2019年第21期4020-4025,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81470815)

摘　　要：目的:探究YY肽(PYY)对雄性Wistar大鼠的摄食、胃运动和能量代谢的影响及潜在机制。方法:采用免疫组织化学实验方法观察大鼠下丘脑弓状核(ARC)中Y2受体的表达;通过ARC微量注射PYY,观察其对下丘脑中编码摄食相关代谢激素的m RNA表达以及ARC中PYY反应性神经元的放电频率、食物摄入量及水摄入量、氧气消耗(VO2)、CO2产生(VCO2)及能量代谢的影响。结果:免疫组化结果显示大鼠ARC内存在Y2受体;大鼠ARC注射PYY能够兴奋PYY反应性神经元,上调可卡因-苯丙胺调节转录肽(CART)及促肾上腺皮质释放激素(CRH)等抑食肽m RNA的表达,下调神经肽Y(NPY)及下丘脑泌素(HCRT)等促食肽m RNA的表达;且抑制大鼠食物摄入量,并参与调控大鼠呼吸、能量代谢及胃运动的改变。结论:ARC微量注射PYY可减少食物摄入并调节全身能量平衡,PYY可能是一种新型代谢肽。Objective: To investigate the effects and potential mechanisms of YY peptide(PYY) on the feeding, gastric motility and energy metabolism in male Wistar rats. Methods: The expression of Y2 receptor in the arcuate nucleus(PYY) of rat hypothalamus was observed by immunohistochemistry. The expression of metabolic hormone m RNA in the hypothalamus and the PYY response in ARC were observed by microinjection of PYY into ARC. Sexual neuron discharge frequency, rat food intake and water intake, oxygen consumption(VO2), CO2 production(VCO2) and energy metabolism. Results: Immunohistochemistry showed that Y2 receptor was present in rat ARC;PYY in rats with ARC could stimulate PYY-reactive neurons;ARC microinjection of PYY could up-regulate the expression of anti-peptide peptides such as(cocaine amphetamine-regulated transcript) CART and(corticotropin releasing hormone) CRH, and down-regulate Neuropeptide Y(NPY) and hypocretin(HCRT). The expression of the peptide peptide m RNA;and PYY can participate in the regulation of changes in rat respiration, energy metabolism and gastric motility. Conclusion: ARC microinjection of PYY can reduce food intake and regulate systemic energy balance. PYY may be a new metabolic peptide in rats.

关 键 词：YY肽 弓状核 摄食 胃运动 能量代谢 

分 类 号：R-33[医药卫生] Q593.2[生物学—生物化学]