辣木多糖提取物对大鼠非酒精性脂肪性肝病保护效果  

Protective effect and mechanism research of Moringa spp. polysaccharide extract on nonalcoholic fatty liver disease in rats

作　　者：罗雪婷 王庆[3] 张菊梅[1,2] 郭伟鹏 胡丹丹[1,2] 丘明泉 LUO Xueting;WANG Qing;ZHANG Jumei;GUO Weipeng;HU Dandan;QIU Mingquan(Guangdong Institute of Microbiology,State Key Laboratory of Applied Microbiology Southern China,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Open Laboratory of Applied Microbiology,GDAS'Project of Science and Technology Development,Guangdong Guangzhou 510070,China;Guangdong Academy of Sciences,Guangdong Guangzhou 510070,China;SUN YAT-SEN University,Guangdong Guangzhou 510275,China)

机构地区：[1]广东省微生物研究所华南应用微生物国家重点实验室广东省菌种保藏与应用重点实验室广东省微生物应用新技术公共实验室,广东广州510070 [2]广东省科学院,广东广州510070 [3]中山大学,广东广州510275

出　　处：《中国食品卫生杂志》2019年第6期517-521,共5页Chinese Journal of Food Hygiene

基　　金：广东省教育部产学研结合项目(2013B091100012);广东省科学院2017年实施创新驱动发展能力建设专项资金项目(2017GDASCX-0201)

摘　　要：目的通过建立非酒精性脂肪性肝病(NAFLD)评价模型,研究辣木多糖提取物预防NAFLD的功效及其作用机制。方法 50只雄性大鼠随机分为阴性对照组、模型对照组以及低、中、高剂量组。各剂量组予以辣木多糖提取物水溶液灌胃(10 ml/kg BW),阴性对照组和模型对照组以同等体积纯水灌胃,每日一次,直至第90天。试验结束后,检测血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、超氧化物歧化酶(SOD)、丙二醛(MDA)、白细胞介素6(IL-6)、白细胞介素1(IL-1)、坏死因子-α(TNF-α)。对所有的肝组织进行组织病理学检查。结果各剂量组与模型对照组体质量比较,差异均有统计学意义(P<0.05)。不同剂量组大鼠血清中抗氧化、抗炎指标含量与模型对照组比较,差异均有统计学意义(P<0.05),且随着剂量的增加,其各指标含量趋向于阴性对照组的含量,血脂指标高剂量组大鼠LDL含量与模型对照组比较差异有统计学意义(P<0.05)。病理镜下发现,各剂量组肝细胞虽有一定程度损伤,但病变种类较模型对照组少,且程度轻,有不同程度的改善。肝细胞脂肪变性程度随着剂量的升高而减轻。结论本研究结果表明,辣木多糖提取物可改善NAFLD的脂质代谢紊乱、降低大鼠血脂水平、对抗氧自由基的攻击、改善炎症反应,保护肝脏。这种保护作用可能是通过抗氧化、抗炎途径而实现的。Objective This study was conducted to investigate the effects and mechanism of Moringa spp. polysaccharide extract on the occurrence and development of nonalcoholic fatty liver disease(NAFLD). Methods Fifty male rats were randomly divided into five groups(10 in each group):negative control group, model control group, low, medium and high dose group. From week 1, the negative control group and the model control group were given 10 ml/kg BW pure water by gavage once a day;each intervention group was given 10 ml/kg BW aqueous solution of Moringa spp. polysaccharide extract by gavage once a day until the 90 th day. Blood samples were taken from the abdominal aorta and liver tissues after sacrifice. The total cholesterol(TC), triglyceride(TG), low-density lipoprotein(LDL), high-density lipoprotein(HDL), alanine aminotransferase(ALT), aspartate aminotransferase(ASL), superoxide dismutase(SOD) and malondialdehyde(MDA) of the serum were tested with the automatic biochemical analyzer. Serum interleukin 6(IL-6), interleukin 1(IL-1) and necrosis factor-α(TNF-α) were determined. Paraffin sections of liver tissue samples were stained with hematoxylin eosin. Results At the end of the experiment, there were statistically significant differences in body weight between the intervention group and the model control group(P<0.05). With the increase of dose, the serum index content(lipids, antioxidants, anti-inflammatory) in the intervention group was significantly different from that in the model control group(P<0.05), and tended to be the same as that in the negative control group. There also were statistically significant differences in LDL content between the high-dose group and the model control group(P<0.05). Microscopically, the model control group shown a variety of intrahepatic lesions, with deep lesions, severe hepatocyte steatosis. Although the hepatocytes in each intervention group were damaged to a certain degree, the types of lesion was less and less severe than that in the model control group. Conclusion The result o

关 键 词：非酒精性脂肪性肝病 辣木多糖提取物 高脂 

分 类 号：R155[医药卫生—营养与食品卫生学]