CDR1as在喉鳞状细胞癌中的表达及临床意义  

Expression and clinic significance of CDR1as in laryngeal squamous cell carcinoma

作　　者：张建中[1] 赵耀新[1] 胡华勇[1] 王燕玲 黄海琼 蔡刚[1] ZHANG Jian-zhong;ZHAO Yao-xin;HU Hua-yong;WANG Yan-ling;HUANG Hai-qiong;CAI Gang(Department of Otorhinolaryngology,the Fifth Hospital Affiliated to Guangzhou Medical University,Guangzhou 510700,China)

机构地区：[1]广州医科大学附属第五医院耳鼻咽喉科

出　　处：《中国耳鼻咽喉颅底外科杂志》2019年第6期645-649,共5页Chinese Journal of Otorhinolaryngology-skull Base Surgery

基　　金：广东省医学科研基金项目(B2018199)

摘　　要：目的探讨环状RNA ciR-7(CDR1as)在喉鳞状细胞癌(laryngeal squamous cell carcinoma,LSCC)中的表达及临床意义,并分析CDR1as与miR-7的相关性。方法收集2011-2013年手术切除并病理确诊为鳞状细胞癌的标本61例,同期选取病理确诊为炎症或正常黏膜的癌旁组织(距肿瘤切缘大于0.5 cm)标本48例,采用实时荧光定量PCR方法检测喉癌及癌旁组织中CDR1as、miR-7的转录表达水平。结果Real-time PCR显示,在LSCC组织中,CDR1as mRNA表达水平均显著高于癌旁组织(P<0.001);miR-7 mRNA表达水平均显著低于癌旁组织(P<0.0001);CDR1as在淋巴结转移患者的LSCC组织中表达水平较无淋巴结转移患者的LSCC组织中显著增高(P<0.01);CDR1as在不同TNM分期患者的喉癌组织中表达水平差异具有统计学意义(P<0.05);CDR1as在LSCC组织中表达水平与患者的预后相关(P=0.02);同时,CDR1as表达水平与miR-7表达呈负性相关(r=-0.643,P=0.001)。结论CDR1as参与LSCC的发生和发展,其表达可以作为判断预后的一项指标,CDR1as与miR-7呈现出的一定的相关性,CDR1as可能通过靶向调控miR-7的表达影响喉癌进展。Objective To investigate the expression and clinical significance of ci-RNA ciR-7(CDR1as)in laryngeal squamous cell carcinoma(LSCC),and to analyze the correlation between CDR1as and miR-7.Methods From 2011 to 2013,real-time fluorescence quantitative polymerase chain reaction(RT-PCR)was used to detect the transcriptional expression levels of CDR1as and miR-7 in 61 specimens of laryngeal squamous cell carcinoma and 48 of normal tissues adjacent to tumor.Results RT-PCR showed that the expression of CDR1as in the LSCC tissues was significantly higher than that in the adjacent tissues(P<0.001),the expression of miR-7 in the LSCC tissues was significantly lower than that in the adjacent tissues(P<0.0001),the expression of CDR1as in LSCC tissues from patients with lymph node metastasis was significantly higher than that from those without lymph node metastasis(P<0.01),and the expression level of CDR1as in the LSCC tissues of patients with different TNM stages were significantly different(P<0.05).The difference of 5-year survival rate between patients with high expression level of CDR1as in the LSCC tissues and those with low expression level was statistically significant(P=0.02),meanwhile,the expression level of CDR1as was negatively correlated with that of miR-7(r=-0.643,P=0.001).Conclusions CDR1as is involved in the occurrence and development of LSCC,and its expression can be used as a prognostic indicator of this tumor.CDR1as has a certain correlation with miR-7.CDR1as may affect the progression of LSCC by targeted-regulating the expression of miR-7.

关 键 词：喉鳞状细胞癌 CDR1as miR-7 预后 

分 类 号：R739.65[医药卫生—肿瘤]