移植NAFLD患者和健康人粪菌对高脂饮食大鼠肠道菌群的影响  

Effects of fecal bacteria transplantation on microflora in rats with high-fat-induced liver steatosis

作　　者：骆庆玲 周永健[1] 唐文娟 首第文 黄红丽[1] 徐豪明 黄惠康[1] 何杰[1] 杜艳蕾[1] 阮焕梅 陈慧婷[1] 曹创裕 Luo Qingling;Zhou Yongjian;Tang Wenjuan(Department of Gastroenterology,First People’s Hospital,Guangzhou Medical University,Guangzhou 510030,Guangdong Province,China)

机构地区：[1]广州医科大学附属广州市第一人民医院消化科

出　　处：《实用肝脏病杂志》2020年第1期134-137,共4页Journal of Practical Hepatology

基　　金：广州市医药卫生科技项目(编号:20181A011007);广州市卫生健康科技项目(项目编号:20191A011001);广州市卫生和计划生育科技项目(20181A011006);广东省医学科学技术研究基金项目(项目编号:20171025211834334)

摘　　要：目的探讨粪菌移植干预对非酒精性脂肪性肝病(NAFLD)动物肠道菌群的影响。方法将32只大鼠随机分为ABCD四组,其中3组用混合抗生素抑菌1周后,予以高脂饮食建立NAFLD模型,再分别给予NAFLD患者粪菌或和正常人粪菌移植,检测肠粘膜组织16sDNA菌群。结果模型组Rodentibacter菌群丰度较对照组升高,在NAFLD粪菌移植组升高更显著,而在正常人粪菌移植组则有所下降。结论NAFLD粪菌群可能是肝脏脂肪变性的一个危险因素,而应用健康人粪菌移植可以改善高脂饮食大鼠脂肪肝病变。Objective The aim of this study was to investigate the effects of fecal bacteria transplantation(FBT)on microflora in rats with high-fat-induced liver steatosis.Methods 32 rats were randomly divided into group A,B,C and D.The non-alcoholic fatty liver disease(NAFLD)model was established by high-fat feeding,and fecal bacteria transplantation from patients with NAFLD and healthy persons were performed.The 16 sDNA flora of intestinal mucosal tissue were analyzed.Results The content of Rodentibacter in the model group was increased as compared to that in the control,but it increased more greatly in the rats with FBT from NAFLD,and it decreased greatly in rats with FBT from healthy persons.Conclusion The bacteria flora of patients with NAFLD might be a risk factor for hepatic steatosis,and FBT by using healthy person’s feces might alleviate the liver steatosis.

关 键 词：非酒精性脂肪性肝病 肠道菌群 粪菌移植 菌群丰度 大鼠 

分 类 号：R575.5[医药卫生—消化系统]