脐带组织移植对辐射诱导大鼠学习记忆损伤的保护作用及机制  

Protective effect and mechanisms of umbilical cord tissue transplantation on radiation-induced learning and memory impairment in rats

作　　者：朱泽军 赵阳 张爱国 陈乃耀 赵辉[3] 白娣斯 张秀军 Zhu Zejun;Zhao Yang;Zhang Aiguo;Chen Naiyao;Zhao Hui;Bai Disi;Zhang Xiujun(College of Psychology and Mental Health,North China University of Science and Technology,Tangshan 063210,China;Pharmacy of North China University of Technology Hospital,Tangshan 063000,China;Internal Medicine of North China University of Technology Hospital,Tangshan 063000,China;School of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China)

机构地区：[1]华北理工大学心理与精神卫生学院,唐山063210 [2]华北理工大学附属医院,唐山063000 [3]天津商业大学生物技术与食品科学学院,300134

出　　处：《中华行为医学与脑科学杂志》2020年第1期8-13,共6页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目(31571832);河北省中医药管理局项目(2017210);天津市创新团队项目项目(TD-13-5087);唐山市脑与认知功能研究基础创新团队项目(17130204D)。

摘　　要：目的探讨脐带组织移植对大鼠辐射诱导的学习记忆损伤的保护作用及其机制。方法将60只SD大鼠随机分为3组,每组20只,分别为对照组、模型组(全脑X射线照射,剂量20 Gy)、治疗组(全脑X射线照射,剂量20 Gy+脐带组织移植)。观察大鼠体质量变化,并于照射后第14天、第28天利用水迷宫实验观察大鼠学习记忆情况,HE染色观察大鼠海马区神经元状态;Western blot检测海马NF-κB通路相关蛋白及IL-6的表达情况。采用SPSS 17.0进行描述性分析和假设检验。结果(1)水迷宫实验中第28天治疗组逃避潜伏期明显高于对照组且低于模型组[对照组:(11.77±3.02)s,模型组:(23.75±3.27)s,治疗组:(18.49±2.32)s;t=3.940,-2.943,均P<0.05)];第28天大鼠在穿越平台次数上治疗组明显低于对照组且高于模型组[对照组:(7.20±0.84)次,模型组:(3.60±1.14)次,治疗组:(5.00±1.00)次;t=-3.773,2.064,均P<0.05)]。(2)HE染色发现对照组大鼠神经元排列整齐,胞浆透明;模型组大鼠部分神经元排列紊乱,胞体收缩呈三角形或不规则形;治疗组大鼠神经元变性坏死和缺失的数量较模型组减轻。(3)第14天TLR4的相对表达量治疗组明显高于对照组且低于模型组[对照组:(0.69±0.03),模型组:(1.06±0.11),治疗组:(0.90±0.04);t=7.275,-2.368,均P<0.05)]、NF-κB P65的相对表达量治疗组明显高于对照组且低于模型组[对照组:(1.67±0.12),模型组:(2.08±0.06),治疗组:(1.93±0.08);t=3.236,-2.684,均P<0.05)]。治疗组IL-6的相对表达量明显高于对照组且低于模型组[对照组:(0.77±0.08),模型组:(1.12±0.07),治疗组:(0.95±0.06);t=3.274,-3.495,均P<0.05)];治疗组BCL-2/Bax的相对表达量显著低于对照组且高于模型组[对照组:(1.40±0.52),模型组:(0.48±0.06),治疗组:(0.72±0.03);t=-2.263,6.350,均P<0.05)];第28天IL-6、BCL-2/Bax蛋白的表达趋势与第14天相同。结论脐带组织移植可以改善由放疗引起的学习记忆障碍,这可能与抑制放疗引Objective To investigate the protective effect and mechanisms of umbilical cord tissue transplantation on radiation-induced learning and memory impairment in rats.Methods Sixty SD rats were randomly divided into three groups with 20 in each group:control group,model group(whole brain X-ray irradiation,dose 20 Gy)and treatment group(whole brain X-ray irradiation,dose 20 Gy+umbilical cord tissue transplantation).The changes of body mass were observed,and the learning and memory of rats were observed by water maze test on the 14th and 28th day after irradiation,the neuron state of hippocampus was observed by HE staining,and the expressions of NF-κB pathway related proteins and IL-6 in hippocampus were detected by Western blot.Descriptive analysis and hypothesis testing were processed by SPSS 17.0.Results(1)On the 28th day,the escaping latency in the water maze experiment of the treatment group was significantly higher than that of the control group and lower than that of the model group(control group:(11.77±3.02)s,model group:(23.75±3.27)s,treatment group:(18.49±2.32)s;t=3.940,-2.943,both P<0.05);the number of crossing platform in the treatment group was significantly lower than that in the control group and higher than that in the model group(control group:(7.20±0.84),model group(3.60±1.14),treatment group(5.00±1.00);t=-3.773,2.064,both P<0.05).(2)HE staining showed that the neurons in the control group were arranged orderly and the cytoplasm was transparent.The neurons in the model group were arranged disorderly and the contraction of the cell body was triangular or irregular.The number of neurons in the treatment group was less than that in the model group.(3)On the 14th day,the relative expression of TLR4 in the treatment group was significantly higher than that in the control group and lower than that in the model group(control group:(0.69±0.03),model group:(1.06±0.11),treatment group:(0.90±0.04);t=7.275,-2.368,both P<0.05).The relative expression of NF-κB p65 in the treatment group was significantly

关 键 词：脐带组织 脐带间充质干细胞 放疗 炎症 学习与记忆 

分 类 号：R[医药卫生]