Efficacy of Banxia Xiexin decoction in a rat model of chronic atrophic gastritis  

作　　者：Bai Yu Chen Yongcan Chen Yu Yan Maoxiang Wei Hongquan Wang Hengcang Zeng Guoliang Yang Xiyin 

机构地区：[1]Department of Internal Medicine of Traditional Chinese Medicine,Zhejiang Academy of Traditional Chinese Medicine,Hangzhou 310007,China [2]Department of Experimental Animal Center,Zhejiang Academy of Traditional Chinese Medicine,Hangzhou 310007,China [3]Department of Second Center Laboratory,Zhejiang Provincial Hospital of Traditional Chinese Medicine,Hangzhou 310006,China [4]Department of Pathology,Zhejiang Academy of Traditional Chinese Medicine,Hangzhou 310007,China

出　　处：《Journal of Traditional Chinese Medicine》2019年第6期867-874,共8页中医杂志（英文版）

基　　金：Supported by the Natural Science Foundation of Zhejiang Province(Notch signaling-based Mechanistic Studies on the Therapeutic Role of Banxia Xiexin Decoction in Chronic Atrophic Gastritis,No.LY15H270013)

摘　　要：OBJECTIVE:To investigate the effectiveness of Banxia Xiexin decoction(BXD)in a rat model of chronic atrophic gastritis(CAG).METHODS:Sixty 6-week-old healthy Wistar rats(30 males,30 females)were used in the present study.A rat model of CAG was successfully established using the combined active immunization/ethanol/sodium deoxycholate method.BXD was prepared from a mixture of seven Chinese herbs,and was intragastrically administered to CAG rats at three different doses(6,12,and 24 g·kg^-1·d^-1).Af-ter 24 weeks,the rats were euthanized,and gastric tissue specimens were collected.Gastric mucosal specimens were stained with hematoxylin and eosin for histological examination to evaluate the degree of inflammation and morphological changes.Immunohistochemical staining was performed to examine the mucosal expression of proliferating cell nuclear antigen.Serum gastrin levels were measured using radioimmunoassay.The expression of Notch signaling-associated genes was examined by quantitative polymerase chain reaction and Western blot assay.RESULTS:BXD at all three doses significantly reversed the adverse effects generated by CAG in rats.Compared with control rats,the CAG rats who were administered BXD had an accelerated growth rate,reduced inflammatory cell infiltration,improved gastric mucosal morphology,augmented thickness of the gastric mucosa,increased number of gastric glands,enhanced mucosal expression of proliferating cell nuclear antigen,and elevated serum gastrin levels.OBJECTIVE: To investigate the effectiveness of Banxia Xiexin decoction（BXD） in a rat model of chronic atrophic gastritis（CAG）.METHODS: Sixty 6-week-old healthy Wistar rats（30 males, 30 females） were used in the present study. A rat model of CAG was successfully established using the combined active immunization/ethanol/sodium deoxycholate method. BXD was prepared from a mixture of seven Chinese herbs,and was intragastrically administered to CAG rats at three different doses(6, 12, and 24 g·kg-1·d-1). Af-ter 24 weeks, the rats were euthanized, and gastric tissue specimens were collected. Gastric mucosal specimens were stained with hematoxylin and eosin for histological examination to evaluate the degree of inflammation and morphological changes.Immunohistochemical staining was performed to examine the mucosal expression of proliferating cell nuclear antigen. Serum gastrin levels were measured using radioimmunoassay. The expression of Notch signaling-associated genes was examined by quantitative polymerase chain reaction and Western blot assay.RESULTS: BXD at all three doses significantly reversed the adverse effects generated by CAG in rats. Compared with control rats, the CAG rats who were administered BXD had an accelerated growth rate, reduced inflammatory cell infiltration, improved gastric mucosal morphology, augmented thickness of the gastric mucosa, increased number of gastric glands, enhanced mucosal expression of proliferating cell nuclear antigen, and elevated serum gastrin levels.

关 键 词：Gastritis atrophic Receptors Notch GASTRINS INFLAMMATION Banxia Xiexin decoction 

分 类 号：R285.5[医药卫生—中药学]