密蒙花滴眼液对去势雄兔干眼结膜炎性因子IL-1β和黏蛋白5AC及P38MAPK表达的影响  

Effects of butterflybush flower eye drops on expression of inflammatory cytokines IL-1β,Mucin 5AC and P38MAPK in castrated male rabbits

作　　者：彭晓芳 彭清华[1] 彭俊[3] 周亚莎 张又玮 覃艮艳 Xiao-Fang Peng;Qing-Hua Peng;Jun Peng;Ya-Sha Zhou;You-Wei Zhang;Gen-Yan Qin(Hunan University of Chinese Medicine,Changsha 410208,Hunan Province,China;The Traditional Chinese Medicine Hospital of Changsha/The No.8 Hospital of Changsha,Changsha 410100,Hunan Province,China;Department of Ophthalmology,the First Hospital of Hunan University of Chinese Medicine,Changsha 410007,Hunan Province,China)

机构地区：[1]湖南中医药大学,湖南省长沙市410208 [2]长沙市中医医院长沙市第八医院,湖南省长沙市410100 [3]湖南中医药大学第一附属医院眼科,湖南省长沙市410007

出　　处：《国际眼科杂志》2020年第3期426-431,共6页International Eye Science

基　　金：国家自然科学基金面上资助项目(No.30772824,81574031);湖南省高层次卫生人才“225”工程培养项目资助[No．湘卫人发(2013)13号];湖南省科技厅科研基金资助项目(No.2015SF2016-6);湖南省发展改革委科研基金资助项目(No．湘发改投资[2014]658号);长沙市科技计划重大专项(No.K1501014-31);湖南中医药大学研究生创新项目(No.2016CX19);湖南中医药大学中医学国内一流建设学科[No．湘教通(2018)469号];国家中医药管理局中医眼科学重点学科建设项目[No．国中医药发(2009)30号];中医药防治眼耳鼻喉疾病湖南省重点实验室建设项目(No.2017TP1018);湖南省中医药防治眼耳鼻咽喉疾病与视功能保护工程技术研究中心(No.2018TP2008)~~

摘　　要：目的:观察不同浓度的密蒙花滴眼液对去势雄兔干眼结膜炎性因子IL-1β、黏蛋白5AC(MUC5AC)及P38MAPK表达的影响,从而探讨密蒙花滴眼液对干眼的疗效。方法:将36只雄兔随机分为:空白组、模型组、密蒙花滴眼液低浓度组(1mg/mL)、中浓度组(1.5mg/mL)、高浓度组(3mg/mL)、睾酮组。除空白组外各组均行睾丸及附睾切除建立干眼动物模型,造模成功后,空白组和模型组不予以处理,密蒙花滴眼液低浓度组(1mg/mL)、中浓度组(1.5mg/mL)、高浓度组(3mg/mL)给予密蒙花不同浓度滴眼液,3次/d,睾酮组按0.5mL/kg剂量在大腿肌肉处注射丙酸睾酮,每3d 1次,各组分别于造模前和用药4wk后在全身麻醉下检测荧光素染色、SchirmerⅠ试验(SⅠt)和泪膜破裂时间(BUT)。用药4wk后处死兔子,取其双眼球结膜组织,采用免疫组织化学染色法检测结膜中IL-1β、MUC5AC、P38MAPK的表达。结果:密蒙花滴眼液低浓度组、中浓度组、高浓度组用药4wk后,SⅠt值明显高于模型组,BUT值明显长于模型组,角膜荧光素染色阳性较模型组有明显改善(均P<0.01)。密蒙花滴眼液低浓度组、中浓度组、高浓度组结膜IL-1β、P38MAPK的阳性表达低于模型组,MUC5AC的表达高于模型组(P<0.01),密蒙花滴眼液高浓度组优于低浓度、中浓度组。结论:密蒙花滴眼液具有类雄激素效果,对于去势雄兔干眼可以下调干眼结膜组织中IL-1β、P38MAPK的表达,增加MUC5AC的表达,从而减少干眼结膜组织的炎症浸润,维持泪膜稳定性,但其作用弱于雄激素。密蒙花滴眼液中、高浓度组对于干眼治疗作用强于低浓度组,高浓度组优于中浓度组。AIM:To observe the effects of butterflybush flower eye drops at different concentrations on expression of inflammatory crtokines IL-1β,Mucin 5AC(MUC5AC)and P38MAPK in castrated male rabbits,and to explore the therapeutic effect of that drops on dry eyes.METHODS:Thirty-six male rabbits were randomly divided into blank group(A),model group(B),low concentrations butterflybush flower eye drops group(C,1mg/mL),the medium concentrations drops group(D,1.5mg/mL),the high concentrations drops group(E,3mg/mL),and testosterone group(F).In addition to group A,the testes and epididymis were removed from each group to establish a dry eye animal model.After successful modeling,groups A and B remain unchanged.Groups C,D,and E were given different concentrations of butterflybush flower eye drops,3 times/d.In group F,testosterone propionate was injected into the muscles of the thigh at a dose of 0.5mL/kg once every 3d.Fluorescein staining,Schirmer I test(SⅠt)and tear film break time(BUT)were measured under general anesthesia in each group,eatment.After 4wk of treatment,the rabbits were sacrificed and the conjunctival tissues of the eyes were taken.The expression of IL-1β,mucin 5AC and P38MAPK in the conjunctiva was detected by immunohistochemical staining.RESULTS:Among low concentrations butterflybush flower eye drops group,the medium concentrations drops group and the high concentrations drops group,the SⅠt value was significantly higher than that of model group,and BUT was significantly longer than model group.The positive staining of corneal fluorescein was significantly improved compared with model group,which was statistically significant(P<0.01).Among IL-1βand P38MAPK in the conjunctiva of high concentrations butterflybush flower eye drops group,the medium concentrations drops group and the low concentrations drops group,the positive expressions were lower than those in model group,and the expression of MUC5AC was higher than that in group model group(P<0.01).In addition,the high concentrations drops group was superio

关 键 词：干眼 密蒙花滴眼液 白介素-1β 黏蛋白5AC P38丝裂原激活蛋白激酶 

分 类 号：R777.34[医药卫生—眼科] R-332[医药卫生—临床医学]