嗅觉剥夺对小鼠大脑桶状皮层谷氨酸能神经元动作电位及Ankyrin-G表达的影响  

Effects of olfactory deprivation on action potential and ankyrin-G expression in glutamatergic neurons in the barrel cortex of mice

作　　者：倪虹[1] 丁海虎[1] 陶静[1] 王元元[1] 陶明飞[1] 黄丽[2] NI Hong;DING Haihu;TAO Jing;WANG Yuanyuan;TAO Mingfei;HUANG Li(Department of Functional Experiment Center,Bengbu Medical College,Bengbu 233030,China;Department of Pathophysiology,Bengbu Medical College,Bengbu 233030,China)

机构地区：[1]蚌埠医学院机能实验中心,安徽蚌埠233030 [2]蚌埠医学院病理生理学教研室,安徽蚌埠233030

出　　处：《南方医科大学学报》2020年第2期262-267,共6页Journal of Southern Medical University

基　　金：安徽高校自然科学研究项目(KJ2017A243,KJ2019A0324);安徽省蚌埠医学院自然科学基金重点项目(BYKY1620ZD)

摘　　要：目的探讨嗅觉剥夺触觉功能上调小鼠模型大脑桶状皮层谷氨酸能神经元动作电位及Ankyrin-G表达变化的相关性。方法12 d龄雄性小鼠40只予氯仿40μL滴入小鼠一侧鼻腔,破坏小鼠一侧嗅觉,复制嗅觉剥夺小鼠模型。实验分为2组,对照组和嗅觉剥夺组。其中嗅觉剥夺模型小鼠的嗅觉剥夺侧脑区为嗅觉剥夺组,对侧脑区为对照组。行为学实验检测嗅觉剥夺对小鼠触觉,即触须功能的影响,如小鼠触须功能增强表明模型建立成功。模型建立成功后,膜片钳电生理记录小鼠大脑桶状皮层谷氨酸能神经元动作电位发放及免疫组织化学法测量其轴突近端Ankyrin-G表达的变化。结果与对照组相比,嗅觉剥夺组小鼠谷氨酸能神经元动作电位发放表现为峰间距缩短和绝对不应期降低(P<0.01),桶状皮层谷氨酸能神经元轴突近端Ankyrin-G表达量增加(P<0.01)。结论嗅觉剥夺可诱导小鼠触觉功能上调,其发生发展机制可能与大脑桶状皮层谷氨酸能神经功能增强及Ankyrin-G表达增加有关,这可能是引发小鼠触觉敏感性增加的机制之一。Objective To investigate effect of upregulated touch sensation induced by olfactory deprivation on action potentials and ankyrin-G expression in the glutamatergic neurons in the barrel cortex of mice.Methods Chloroform(40μL)was dropped into the unilateral nasal cavity to induce olfactory deprivation in 40 C57 mice(12 days old),whose glutamatergic neurons were genetically labeled with yellow fluorescent protein(YFP).Behavioral experiments were carried out to assess the effects of olfactory deprivation on whisker tactile of the mice.The action potentials of the glutamatergic neurons in the barrel cortex on the side with or without chloroform treatment(olfactory deprivation group and control group,respectively)were recorded by patch-clamp electrophysiological recording,and ankyrin-G expression in the proximal axonal segment of the neurons was detected with immunohistochemistry.Results Compared with those on the control side,the inter-spike intervals of the barrel glutamatergic neurons were significantly decreased and the absolute refractory periods were significantly shortened on the side with olfactory deprivation(P<0.01);the expression of ankyrin-G was also significantly increased in the proximal axonal segment of the glutamatergic neurons in the barrel cortex on the side with olfactory deprivation(P<0.01).Conclusion Olfaction deprivation induces up-regulation of touch sensation in mice possibly as a result of functional enhancement of the glutamatergic neurons and increased ankyrin-G expression in the barrel cortex.

关 键 词：嗅觉剥夺 桶状皮层 谷氨酸能神经元 全细胞记录 动作电位 ANKYRIN-G 

分 类 号：R338[医药卫生—人体生理学]