GC-MS法同时测定抗草甘膦转基因大豆中多种除草剂的残留量  

Simultaneous Determination of Herbicide Residues in Glyphosate Resistant Transgenic Soybeans by GC-MS

作　　者：何龙凉 陈延伟 李小琴 杨怀军 韦新红 

机构地区：[1]中华人民共和国防城海关

出　　处：《大众科技》2019年第12期17-19,共3页Popular Science & Technology

摘　　要：利用气相色谱-质谱联用法(Gas Chromatography-tandem Mass Spectrometry,GC-MS)同时测定防城港口岸进境的不同原产国抗草甘膦转基因大豆(Roundup Ready Soybean,简称RR大豆)中氟乐灵、甲草胺、异丙甲草胺、吡氟禾草灵、烯禾啶的残留量。方法为采集原产自美国、巴西、阿根廷和乌拉圭的RR大豆各3批,样品磨碎过筛以乙腈(含1%乙酸)为萃取剂用加速萃取仪进行萃取,萃取液过Envi-18固相萃取柱,再经Sep-Pak Carbon/NH2双层柱进一步净化后,加入20μL环氧七氯内标溶液,用正己烷定容至1 mL供GC-MS分析。结果表明,氟乐灵、甲草胺、异丙甲草胺、吡氟禾草灵和烯禾啶在不同浓度范围内的线性良好(r>0.99),样品加标平均回收率为77.4%~92.5%,相对标准偏差为4.5%~10.1%,定量下限0.01~0.05 mg/kg。该分析方法,方法准确可靠,回收率和精密度符合残留检测要求,对提高进口转基因大豆监管工作效率和进一步分析评估转基因大豆的食用安全性有一定的参考价值。Gas Chromatography-Tandem Mass Spectrometry(GC-MS)was used to simultaneously determine the residues of trifluralin,metolachlor,metolachlor,picroflofenac,and echloridine in Roundup Ready Soybean(RR soybean)from different countries of origin that had entered Fangchenggang port.Methods:Three batches of RR soybeans from the United States,Brazil,Argentina and Uruguay were collected.The samples were ground and sieved with acetonitrile(containing 1%acetic acid)as extractant and extracted with an accelerated extractor.The extract was passed through the Envi-18 solid phase extraction column.The column was further purified by Sep-Pak Carbon/NH2 double-layer column,20μL of epoxy heptachlor standard solution was added,and the volume was adjusted to 1 m L with n-hexane for GC-MS analysis.Results:The linearity of trifluralin,alachlor,metolachlor,pyridoxine and methotrexate in different concentration ranges was good(r>0.99).The average recovery of standard addition was 77.4%~92.5%.The relative standard deviation is 4.5%to 10.1%,and the lower limit of quantification is 0.01~0.05 mg/kg.The analysis method is accurate and reliable,the recovery rate and precision meet the requirements of residue detection.It has certain reference value for improving the supervision efficiency of imported genetically modified soybeans and further analyzing and evaluating the edible safety of genetically modified soybeans.

关 键 词：抗草甘膦转基因大豆 气相色谱-质谱联用 除草剂 

分 类 号：O657[理学—分析化学]