MDM2/p53通路对多发性骨髓瘤RPMI 8226细胞凋亡的调控  

The role of MDM2/p53 pathway in regulating apoptosis of multiple myeloma RPMI8226 cells

作　　者：梁昊[1] 廖灿 李红[3] 刘文龙[4] LIANG Hao;LIAO Can;LI Hong;LIU Wenlong(Institute of TCM Diagnostics,Hunan University of Chinese Medicine,Changsha 410208;School of Chinese Medicine,Hunan University of Chinese Medicine,Changsha 410208;Science and Technology Innovation Center,Hunan University of Chinese Medicine,Changsha 410208;School of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学中医诊断研究所,湖南长沙410208 [2]湖南中医药大学中医学院,湖南长沙410208 [3]湖南中医药大学科技创新中心,湖南长沙410208 [4]湖南中医药大学药学院,湖南长沙410208

出　　处：《西安交通大学学报（医学版）》2020年第2期197-200,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：中医内科重大疾病防治及成果转化教育部重点实验室开放基金资助项目(No.ZYNK201607);大学生研究性学习和创新性实验计划项目(No.2018-01);国家自然科学基金资助项目(No.81874344);中国博士后基金项目(No.2018M640755);湖南省自然科学基金面上项目(No.2019JJ0307)~~

摘　　要：目的研究MDM2/p53通路对多发性骨髓瘤(MM)细胞凋亡的调控作用及机制。方法体外培养人MM细胞株(RPMI 8226)并分为实验组(Nutlin-3a)和对照组(药物溶剂),采用CCK-8法检测各组细胞的增殖情况和抑制率,Western blot检测凋亡相关蛋白表达,流式细胞术检测细胞凋亡情况。结果与对照组相比,24、48、72 h时间点实验组细胞增殖明显降低,抑制率明显增高(P<0.05);实验组MDM2和Bcl-2蛋白表达明显降低(P<0.05),而p53的表达水平明显升高(P<0.05)。实验组各时间点凋亡率(38.42%、82.26%、82.74%)均明显高于对照组(4.80%、8.06%、14.69%)。结论MDM2与p53之间构成降解-反式激活循环通路影响下游凋亡相关蛋白Bcl-2的表达,抑制MM细胞的增殖,促进MM细胞凋亡。Objective To study the regulatory effect and mechanism of MDM2/p53 pathway on apoptosis in multiple myeloma(MM).Methods RPMI8226 cells were cultured in vitro and divided into Nutlin-3a group,control group and blank group.CCK-8 method was used to detect the cell inhibition rate,Western blot was used to detect the expression of apoptosis-related protein,and flow cytometry was used to detect the apoptosis in the two groups.Results Compared with that in control group,the inhibition rate of cells in the experimental group increased significantly at 24 h,48 h and 72 h(P<0.05).Compared with those in the control group,the expression levels of MDM2 and Bcl-2 in the experimental group decreased significantly(P<0.05),while the expression level of p53 increased significantly(P<0.05).The apoptosis rate was significantly higher in the experimental group than in the control group at 24 h,48 h and 72 h(38.42%,82.26%and 82.74%vs.4.80%,8.06%and 14.69%).Conclusion The degradation trans-activation pathway between MDM2 and p53 affects the expression of Bcl-2,inhibits the proliferation of MM cells,and promotes the apoptosis of MM cells.

关 键 词：MDM2 p53 多发性骨髓 凋亡 Nutlin-3a 

分 类 号：R733.3[医药卫生—肿瘤]