CTC检测在胃癌结直肠癌预后的价值和差异性分析  

Difference of CTC detection in the prognosis of gastric cancer and colorectal cancer

作　　者：赵琳 刘医辉 董春慧 张文文 段学琴 刘锡枝 陈玲[1] ZHAO Lin;LIU Yihui;DONG Chunhui;ZHANG Wenwen;DUAN Xueqin;LIU Xizhi;CHEN Ling(Department of Oncology,The First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061;Department of Oncology,People's Hospital of Ningxia Hui Autonomous Region,Yinchuan 750000;Department of Oncology,Ninth Hospital of Xi'an,Xi'an 710054,China)

机构地区：[1]西安交通大学第一附属医院肿瘤科,陕西西安710061 [2]宁夏回族自治区人民医院肿瘤科,宁夏银川750000 [3]西安市第九医院肿瘤科,陕西西安710054

出　　处：《西安交通大学学报（医学版）》2020年第2期263-267,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的探讨外周血循环肿瘤细胞(CTC)检测在胃癌结直肠癌预后的价值和差异。方法采用阴性富集联合免疫荧光原位杂交(imFISH)检测平台检测32例胃癌和36例结直肠癌患者CTC数,并进行组间比较。结果胃癌组患者外周血中检出CTC<2组8例(25%),随访结束时,全部患者存活;检出CTC≥2组有24例(75%),仅有8例存活。两组的生存差异具有统计学意义(P=0.001)。在结直肠癌组患者中外周血检出CTC<2组28例(78%),随访时间结束时,全部患者存活;检出CTC≥2组有8例(22%),其中6例患者在随访终点时间存活。结直肠癌中CTC<2组与CTC≥2组的生存差异具有统计学意义(P=0.044)。胃癌与结直肠癌的生存差异具有统计学意义(P<0.001)。CTC检出结果与胃癌结直肠癌患者的性别、年龄、淋巴结转移、远处器官转移、病理分级均无相关性(均P>0.05)。结论采用阴性富集和imFISH技术,检测胃癌和结直肠癌患者外周血CTC,CTC值升高的患者预后不良,且胃癌较结直肠癌预后更差。Objective To investigate the clinical significance and difference of peripheral blood circulating tumor cells(CTC)detection in the prognosis of patients with gastric cancer and colorectal cancer.Methods The CTC in the peripheral blood of 32 patients with gastric cancer and 36 patients with colorectal cancer were detected by negative enrichment and imFISH technique,and the results were compared between the two groups.Results There were 8 cases(25%)of CTC<2 in the peripheral blood of gastric cancer patients.At the end of the follow-up,all patients survived;24 cases(75%)of CTC(>2)were detected,and only 8 cases survived.The two groups differed significantly in survival(P=0.001).Twenty-eight cases(78%)of the patients with colorectal cancer were detected CTC<2 groups in the peripheral blood.At the end of the follow-up,all patients survived.Eight cases(22%)of the patients with CTC>2 groups survived at the end of the follow-up.Six patients survived at the end of the follow-up.There was a significant difference between CTC<2 groups and CTC>2 groups in colorectal cancer(P=0.044).The survival in gastric cancer and colorectal cancer was significantly different(P<0.001).The results of CTC were not correlated with gender,age,lymph node metastasis,distant organ metastasis or pathological grade of patients with colorectal cancer and stomach cancer(all P>0.05).Conclusion Negative enrichment and imFISH technique can be used to detect CTC of the peripheral blood of patients with gastric cancer and colorectal cancer.The prognosis of patients with elevated CTC is poor.It is worse in gastric cancer than in colorectal cancer.

关 键 词：胃癌 结直肠癌 循环肿瘤细胞 阴性富集技术 imFISH技术 

分 类 号：R57[医药卫生—消化系统]