喉鳞癌中CircRNA、LncRNA、miRNA调控网络构建及功能研究  

Construction and function of CircRNA,LncRNA and miRNA regulatory networks in laryngeal squamous cell carcinoma

作　　者：王微[1] 孙亚男[1] Wang Wei;Sun Yanan(Department of Otolaryngeal,Head and Neck Surgery,the Second Affiliated Hospital of Harbin Medical University,Heilongjiang Harbin 150081,China)

机构地区：[1]哈尔滨医科大学附属第二医院耳鼻咽喉头颈外科

出　　处：《现代肿瘤医学》2020年第5期698-701,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81772874)

摘　　要：目的:探讨喉鳞状细胞癌(laryngeal squamous cell carcinoma,LSCC)中CircRNA、LncRNA、miRNA之间的共表达调控网络。方法:选取哈尔滨医科大学附属第二医院耳鼻咽喉头颈外科喉鳞状细胞癌患者标本,使用微阵列芯片技术对9对喉癌组织及其对应的癌旁非癌组织进行测序。筛选出差异表达的LncRNA、CircRNA、mRNA,通过TargetScan和miRanda算法对差异表达的RNA进行分析,构建出喉鳞状细胞癌的竞争内源性RNA(endogenous competitive RNA,ceRNA)网络。结果:发现8个LncRNA、6个CircRNA和13个mRNA以miR-107为中心形成共表达调控网络。6个LncRNA、3个CircRNA和20个mRNA以miR-19a-3p为中心形成共表达调控网络。结论:构建CircRNA/LncRNA、miRNA、mRNA之间的调控网络,为喉癌的后续治疗提供参考依据。Objective:To investigate the co-expression regulation network between CircRNA,LncRNA and miRNA in laryngeal squamous cell carcinoma.Methods:The specimens of patients with laryngeal head and neck surgery laryngeal squamous cell carcinoma of the Second Affiliated Hospital of Harbin Medical University were selected,and 9 pairs of laryngeal carcinoma tissues and their corresponding paracancerous non-canceroustissues were sequenced using microarray technology.Differential expressions of LncRNA,CircRNA and mRNA were screened,and the differentially expressed RNAs were analyzed by TargetScan and miRanda algorithm,so as to construct the endogenous competitive RNA(ceRNA)network of laryngeal squamous cell carcinoma.Results:Eight LncRNAs,six CircRNAs and thirteen mRNAs were found to form a co-expression regulatory network centering on miR-107.Six LncRNAs,three CircRNAs and twenty mRNAs formed a co-expression regulatory network centering on miR-19a-3p.Conclusion:The CircRNA/LncRNA,miRNA,mRNA network related to laryngeal squamous cell carcinoma was constructed,which may provide a reference to further study of layyngeal squamous cell carcinoma.

关 键 词：喉鳞状细胞癌 非编码RNA 内源竞争性RNA 调控网络 

分 类 号：R739.65[医药卫生—肿瘤]