miR-21调控程序性细胞死亡4基因的表达对宫颈癌细胞增殖和侵袭能力的影响  

Effect of miR-21 on proliferation and invasion of cervical cancer cells through regulating programmed cell death 4 gene expression

作　　者：贺传勇 吴小莉 解为全 倪荣 柯凤梅 He Chuanyong;Wu Xiaoli;Xie Weiquan;Ni Rong;Ke Fengmei(Department of Obstetrics and Gynecology,Enshi Tujia and Miao Autonomous Prefecture Central Hospital,Hubei Enshi 445000,China)

机构地区：[1]恩施土家族苗族自治州中心医院妇产科

出　　处：《现代肿瘤医学》2020年第5期724-728,共5页Journal of Modern Oncology

基　　金：湖北省自然科学基金资助项目(编号:2014CFC1066)

摘　　要：目的:探讨miR-21调控程序性细胞死亡4基因的表达对宫颈癌细胞增殖和侵袭能力的影响及其机制。方法:qPCR检测miR-21和PDCD4在宫颈癌组织中的表达情况;双荧光素酶实验检测miR-21和PDCD4之间的调控作用;细胞集落实验检测抑制miR-21对宫颈癌细胞增殖能力的影响情况,Transwell侵袭实验检测对宫颈癌细胞侵袭能力的影响情况。结果:与癌旁正常组织相比,miR-21在宫颈癌组织中的表达上调,PDCD4在宫颈癌组织中表达下调;双荧光素酶实验检测miR-21和PDCD4之间的调控关系,miR-21可直接调控PDCD4蛋白的表达及活性情况;抑制miR-21的表达后可以抑制宫颈癌细胞的增殖能力;抑制miR-21的表达水平后,宫颈癌细胞的侵袭能力得到一定程度的抑制。结论:miR-21可以调控PDCD4的表达影响宫颈癌细胞的增殖和侵袭能力。Objective:To investigate the effect of miR-21 on the expression of apoptosis-related gene 4(PD-4)on the proliferation and invasion of cervical cancer cells and its mechanism.Methods:qPCR was used to detect the expression of miR-21 and PDCD4 in cervical cancer.Dual luciferase assay detected the regulatory role of miR-21 and PDCD4.Cell colony assay detected the effect of miR-21 on the proliferation of cervical cancer cells.Transwell invasion test detected invasion ability of cervical cancer cells.Results:The expression of miR-21 was up-regulated in cervical cancer and the expression of PDCD4 was down-regulated in cervical cancer compared with adjacent normal tissues.Dual luciferase assay detected the regulatory relationship between miR-21 and PDCD4,miR-21 can directly regulate the expression of PDCD4 protein and activity.Inhibition of miR-21 expression can inhibit the proliferation of cervical cancer cells.After inhibition of miR-21 expression levels,cervical cancer cell invasion ability was inhibited to a certain extent.Conclusion:miR-21 can regulate the expression of PDCD4 in cervical cancer cell proliferation and invasion ability.

关 键 词：MIR-21 宫颈癌 PDCD4 肿瘤侵袭 

分 类 号：R737.33[医药卫生—肿瘤]