RNA结合蛋白QKI-5对肾癌细胞增殖和细胞周期的影响  

RNA binding protein QKI-5 effect on the proliferation and cell cycle of renal cancer cell

作　　者：张瑞丽[1,2] 窦嫣然 艾尼瓦尔·艾木都拉[2] 熊健[1] 包永星[2] Zhang Ruili;Dou Yanran;Ainiwaer·Aimudula;Xiong Jian;Bao Yongxing(Postdoctoral Workstation,Changji Branch Hospital of the First Affiliated Hospital of Xinjiang Medical University,Xinjiang Changji 831100,China;Cancer Center,the First Affiliated Hospital of Xinjiang Medical University,Xinjiang Urumqi 830054,China.)

机构地区：[1]新疆医科大学第一附属医院昌吉分院博士后工作站,新疆昌吉831100 [2]新疆医科大学第一附属医院肿瘤中心,新疆乌鲁木齐830054

出　　处：《现代肿瘤医学》2020年第6期871-876,共6页Journal of Modern Oncology

基　　金：国家自然科学基金项目(编号:81760452);国家级课题第62批中国博士后基金面上项目二等资助(编号:2017M623338XB)

摘　　要：目的:探讨RNA结合蛋白QKI-5在肾癌中的生物学功能。方法:应用以慢病毒为载体的过表达技术观察过表达QKI-5对肾癌细胞增殖作用的影响及对细胞周期的影响。结果:过表达QKI-5能够抑制肾癌细胞株786-O和A-498细胞的增殖能力、克隆形成能力;流式细胞术结果显示:过表达QKI-5使786-O和A-498细胞产生G1期阻滞,而这种阻滞与周期相关蛋白CyclinD1表达降低及p21、p27表达增高有关。结论:QKI-5在肾癌中发挥着抑癌基因的功能,可以抑制肾癌细胞增殖,并使细胞周期阻滞在G1期。Objective:To analyze the function of QKI-5 in renal cell carcinoma(RCC).Methods:Overexpression technique using lentivirws as a vector detected the effect of overexpressing QKI-5 on proliferation and cell cycle of RCC.Results:In vitro assays revealed that the ectopic expression of QKI-5 effectively inhibited cellular proliferation,resulting in significant inhibition of the cellular growth rate and reduction in the colony forming ability of the cells.Moreover,ectopic expression of QKI-5 in 786-O and A-498 cells significantly increased the proportion of cells in G 1 phase and decreased the proportion in S phase and displayed a reduction in Cyclin D1 and an accumulation of p21 and p27.Conclusion:Our data establish a suppressive role for QKI-5 in RCC tumourigenesis.

关 键 词：RNA结合蛋白QKI-5 肾癌 细胞周期 

分 类 号：R737.12[医药卫生—肿瘤]