miR-29a靶向调控CLDN1抑制肝癌细胞增殖的实验研究  

Experimental study on the inhibitory effect of miR-29a regulatory CLDN1 on the proliferation of hepatocellular carcinoma cells

作　　者：沙娅·玛哈提 迪娜·艾尼瓦尔 肖蕾[1] 张华[1] Shaya·Mahati;Dina·Ainiwaer;Xiao Lei;Zhang Hua(Department of Tumor Center,First Affiliated Hospital of Xinjiang Medical University,Xinjiang Urumqi 830011,China.)

机构地区：[1]新疆医科大学第一附属医院肿瘤中心

出　　处：《现代肿瘤医学》2020年第6期887-891,共5页Journal of Modern Oncology

基　　金：新疆维吾尔自治区自然科学基金(编号:2015211C079)

摘　　要：目的:探讨miR-29a在肝癌组织中的表达水平,及其对人肝癌细胞增殖能力的影响。方法:通过RT-PCR测定肝癌组织及非肝癌组织中miR-29a的表达水平;将携带miR-29a的脂质体miR-29a mimic、空载体miR-NC以及miR-29a抑制剂anti-miR-29a转染入肝癌细胞系HepG2和HLE,通过RT-PCR方法检测miR-29a对肝癌细胞系HepG2、HLE增殖能力影响;通过Targetscan软件预测miR-29a靶基因,采用Western blot及荧光素酶实验验证。结果:与非肝癌组织比较,miR-29a在肝癌组织中表达下调,MTT实验提示:miR-29a高表达组肝癌细胞增殖能力较弱,miR-29a低表达组肝癌细胞增殖能力提高。Targetscan软件提示:CLDN1可能为miR-29a靶基因,Western blot及荧光素酶实验提示:与阴性对照组相比,miR-29a高表达组内CLDN1表达下降,相反miR-29a低表达组内CLDN1表达增加。结论:miR-29a在肝癌细胞及组织中表达下调,并且可能通过调控CLDN1影响肝癌细胞增殖能力。Objective:To explore the expression level of miR-29a in liver cancer tissue and its effect on the proliferation ability of human liver cancer cells.Methods:The expression of miR-29a in hepatocellular carcinoma and non-hepatocellular carcinoma tissues were detected by RT-PCR.miR-29a mimics,empty vector miR-29a-NC and miR-29a inhibitors were transfected into hepatocellular carcinoma HepG2 and HLE cell lines,RT-PCR methods were used to observe the effects of miR-29a on proliferation of hepatocellular carcinoma cell lines HepG2 and HLE.The target genes of miR-29a were predicted by Targetscan software and verified by Western blot and luciferase experiments.Results:Compared with non-hepatocarcinoma tissues,miR-29a was down-regulated in hepatocarcinoma tissues.MTT experiments showed that the proliferation ability of hepatoma cells in the high expression group of miR-29a were weak.The proliferation ability of hepatoma cells in the low expression group of miR-29a were increased.Targetscan software suggested that CLDN1 may be a target gene of miR-29a.Western blot and luciferase assays showed that the expression of CLDN1 was decreased in the high expression group of miR-29a compared with the negative control group,and the expression of CLDN1 was decreased in the low expression group of miR-29a.Conclusion:miR-29a is down-regulated in liver cancer cells and tissues,and may affect the proliferation of liver cancer cells by regulating CLDN1.

关 键 词：肝癌 miR-29a 增殖 转移 CLDN1 

分 类 号：R735.7[医药卫生—肿瘤]