基质金属蛋白酶9在皮肤鳞癌患者血清中的表达及意义  

Expression and significance of matrix metalloproteinases-9 in the serum and tumor tissue ofpatients with cutaneous squamous cell carcinoma

作　　者：王辉[1] 孙怡[1] 王振华[1] 严庆涛[2] 高素美[3] 高建芳 王成东[4] WANG Hui;SUN Yi;WANG Zhenhua;YAN Qingtao;GAO Sumei;GAO Jianfang;WANG Chengdong(Department of Dermatology,Weifang People’s Hospital,Weifang 261041,China;Department of Pediatric Surgery,Weifang People’s Hospital;Department of Pathology,Weifang People’s Hospital;Antenatal Diagnosis Laboratory,Weifang People’s Hospital)

机构地区：[1]潍坊市人民医院皮肤科,山东潍坊261041 [2]潍坊市人民医院小儿外科 [3]潍坊市人民医院病理科 [4]潍坊市人民医院产前诊断实验室

出　　处：《潍坊医学院学报》2019年第6期447-449,共3页Acta Academiae Medicinae Weifang

基　　金：潍坊市卫生计生委科研项目计划课题(课题编号:2016wsjs024);潍坊市科技发展计划项目课题(课题编号:2019YX007)

摘　　要：目的探讨基质金属蛋白酶9(MMP-9)在皮肤鳞癌血清中的表达及其临床意义。方法病理证实皮肤鳞状细胞癌患者75例,以46例健康人血清为对照组。应用酶联免疫吸附试验(ELISA)检测各组血清中MMP-9水平的表达情况。结果皮肤鳞癌组血清MMP-9水平明显高于正常对照组,差异有显著性(P<0.01)。MMP-9水平随着肿瘤厚度的增加和临床分期的变晚呈递增趋势(P<0.01)。侵袭性鳞癌组明显高于原位鳞癌组(P<0.01),而高分化鳞癌、中分化鳞癌和低分化鳞癌各组间比较无明显差异(P>0.05)。有淋巴结转移的皮肤鳞癌组明显高于无淋巴结转移组(P<0.01)。用受试者工作曲线(ROC)法建立血清MMP-9水平对有无淋巴结转移的诊断曲线,其曲线下面积(AUC)值为0.913(95%CI 0.839~0.971),诊断最佳临界点为197.75mg/L。结论 MMP-9在皮肤鳞癌的侵袭转移中起重要作用,血清MMP-9水平与皮肤鳞癌的临床病理特征密切相关,可能成为皮肤鳞癌侵袭性判断及有无淋巴结转移的新血清学标志物。Objective To investigate the expression of matrix metalloproteinase 9(MMP-9) in serum of skin squamous cell carcinoma and its clinical significance.Methods Pathologically confirmed 75 patients with squamous cell carcinoma of the skin,and 46 healthy human’s sera were used as control group.The expression of MMP-9 in serum of each group was detected by Enzyme-linked immunosorbent assay(ELISA).Results The level of serum MMP-9 in the skin squamous cell carcinoma group was significantly higher than the control group,which was statistically different,and MMP-9 levels increased with the increase of skin tumor thickness and clinical stages(P<0.01).MMP-9 levels in the aggressive squamous cell carcinoma group were significantly higher than those in the squamous cell carcinoma in situ group(P<0.01),but there was no significant difference between the groups of highly differentiated,medium differentiated,and poorly differentiated squamous cell carcinoma(P>0.05).MMP-9 levels in patients with lymph node metastasis were significantly higher those without lymph node metastasis(P<0.01).The receiver operating curve(ROC) method was used to establish a diagnostic curve of serum MMP-9 levels versus the presence or absence of lymph node metastasis.The area under the curve(AUC) was 0.913(95% CI 0.839 to 0.971).Conclusion MMP-9 plays an important role in the invasion and metastasis of skin squamous cell carcinoma.The serum MMP-9 level is closely related to the clinicopathological characteristics of skin squamous cell carcinoma and may become a new serological marker for judging the aggressiveness of skin squamous cell carcinoma and whether there is lymph node metastasis.

关 键 词：皮肤鳞状细胞癌 基质金属蛋白酶9 血清 病理学 

分 类 号：R739.5[医药卫生—肿瘤]