Identification of QTL for adult plant resistance to stripe rust in bread wheat line C33  

作　　者：LUO Jiang-tao ZHENG Jian-min WAN Hong-shen YANG Wu-yun LI Shi-zhao PU Zong-jun 

机构地区：[1]Crop Research Institute,Sichuan Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Breeding in Wheat(Southwest),Ministry of Agriculture and Rural Affairs

出　　处：《Journal of Integrative Agriculture》2020年第3期624-631,共8页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31671683);the Youth Foundation of Sichuan Academy of Agricultural Sciences,China(2016QNJJ-008)

摘　　要：Stripe rust,caused by Puccinia striiformis f.sp.tritici,is a serious disease in bread wheat(Triticum aestivum L.).Identification and use of adult plant resistance(APR) resources are important for stripe rust resistance breeding.Bread wheat line C33 is an exotic germplasm that has shown stable APR to stripe rust for more than 10 years in Sichuan Province of China.Here,183 recombinant inbred lines(RILs) derived from the cross between C33 and a susceptible line X440 were genotyped with diversity arrays technology(DArT) markers to identify resistance quantitative trait locus(QTL).Field trials were conducted in five years at Chengdu and Xindu of Sichuan Province,using maximum disease severity(MDS) as stripe rust reaction phenotypes.A total of four quantitative trait loci(QTLs) were detected,respectively designed as QYr.saas-3 AS,QYr.saas-5 AL,QYr.saas-5 BL,and QYr.saas-7 DS,explaining 4.14-15.21% of the phenotypic variances.QYr.saas-5 BL and QYr.saas-7 DS were contributed by C33.However,the level for stripe rust resistance contributed by them was not strong as C33,suggesting the presence of other unidentified QTLs in C33.QYr.saas-7 DS corresponded to Yr18 and QYr.saas-5 BL remains to be formally named.The RIL lines carrying combinations QYr.saas-5 AL,QYr.saas-5 BL,and QYr.saas-7 DS showed comparability resistance with C33.The present study provides resources to pyramid diverse genes into locally adapted elite germplasm to improve the stripe rust resistance of bread wheat.Stripe rust, caused by Puccinia striiformis f. sp. tritici, is a serious disease in bread wheat(Triticum aestivum L.). Identification and use of adult plant resistance(APR) resources are important for stripe rust resistance breeding. Bread wheat line C33 is an exotic germplasm that has shown stable APR to stripe rust for more than 10 years in Sichuan Province of China. Here, 183 recombinant inbred lines(RILs) derived from the cross between C33 and a susceptible line X440 were genotyped with diversity arrays technology(DArT) markers to identify resistance quantitative trait locus(QTL). Field trials were conducted in five years at Chengdu and Xindu of Sichuan Province, using maximum disease severity(MDS) as stripe rust reaction phenotypes. A total of four quantitative trait loci(QTLs) were detected, respectively designed as QYr.saas-3 AS, QYr.saas-5 AL, QYr.saas-5 BL, and QYr.saas-7 DS, explaining 4.14–15.21% of the phenotypic variances. QYr.saas-5 BL and QYr.saas-7 DS were contributed by C33. However, the level for stripe rust resistance contributed by them was not strong as C33, suggesting the presence of other unidentified QTLs in C33. QYr.saas-7 DS corresponded to Yr18 and QYr.saas-5 BL remains to be formally named. The RIL lines carrying combinations QYr.saas-5 AL, QYr.saas-5 BL, and QYr.saas-7 DS showed comparability resistance with C33. The present study provides resources to pyramid diverse genes into locally adapted elite germplasm to improve the stripe rust resistance of bread wheat.

关 键 词：APR DART QTL mapping Triticum aestivum L. 

分 类 号：S435.121.42[农业科学—农业昆虫与害虫防治]