致痫大鼠海马谷氨酸及γ-氨基丁酸在痫性发作后含量变化规律  

Changes of glutamic acid and γ-aminobutyric amino acid content in hippocampus of epileptic rats after epileptic seizure

作　　者：曾春梅 雷丹青 孙兰凤 韦雷 何敏[1] 吴原[1] Zeng Chunmei;Lei Danqing;Sun Lanfeng;Wei Lei;He Min;Wu Yuan(Department of Neurology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021;Experimental Center of Life Sciences Institutes,Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学第一附属医院神经内科,广西南宁530021 [2]广西医科大学生命科学研究院医学科学实验中心,广西南宁530021

出　　处：《南京医科大学学报（自然科学版）》2019年第11期1571-1574,1588,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81760242)。

摘　　要：目的:通过检测戊四氮(pentrazole,PTZ)致痫大鼠海马谷氨酸(glutamic amino acid,Glu)及γ-氨基丁酸(γ-aminobutyric amino acid,GABA)在痫性发作后30 min内的含量变化,研究癫痫的终止机制。方法:通过PTZ皮下注射建立SD雄性大鼠急性痫性发作模型,当大鼠痫性发作达Ⅳ~Ⅴ级时,分别于发作时(立即)及发作后5、10、15、20、25、30 min处死大鼠,断头取海马,采用高效液相色谱法检测海马组织Glu与GABA含量。生理盐水皮下注射作为正常对照组。结果:痫性发作达到Ⅳ~Ⅴ级时,0、5、10、15、20、25 min组海马组织Glu含量较正常对照组明显升高,差异具有统计学意义(P <0.05),之后开始下降,30 min组Glu含量与正常对照组相比差异无统计学意义(P> 0.05);海马组织中GABA含量在5 min时显著增高(P <0.05),随后开始下降,10 min时下降至最低,在15 min时再次缓慢升高,持续至25 min时达到高峰(P <0.05),之后开始下降。结论:痫性发作后Glu与GABA含量存在随时间变化的规律,Glu与GABA之间达到动态平衡,是癫痫发作终止的原因之一。Objective:To observe the changes of glutamic amino acid(Glu)and γ-aminobutyric amino acid(GABA)content in the hippocampus of rats in 30 minutes after an epileptic seizure induced by pentrazole(PTZ),and explore the termination mechanism of epilepsy. Methods:Acute epileptic seizures model of SD male rats were induced by PTZ kindling. The model rats were killed immediately or 5,10,15,20,25 and 30 minutes later when the epileptic seizures occurred and reached the level of Ⅳ~Ⅴ. The heads of the rats were cut and the hippocampuses were taken out for detections of the Glu and GABA contents in the hippocampus using high performance liquid chromatography. A normal control group was established via normal saline hypodermic injection. Results:Compared to the normal control group,the levels of Glu content in the hippocampal tissues were higher(P < 0.05)in the model rats 0,5,10,15,20 or 25 minutes after the epileptic seizure reached the level of Ⅳ~Ⅴ symptoms,and the differences were statistically significant(P < 0.05). The Glu content in the 25 min group indicated the peak value,followed by a decline. The Glu content has no significantly difference between the 30 min group and the normal control group(P > 0.05). The level of GABA content rose significantly in the 5 min group(P < 0.05),and subsequently began to decline,it came to the minimum value in the 10 min group,but began to rise slowly and progressively in the 15,20 and 25 min group,and to reach its peak(P < 0.05),then followed by a decline.Conclusion:The research reveals the change rules of Glu and GABA contents over the time after epileptic seizure,and a dynamic balance between the Glu and GABA contents is one of the factors that lead to the termination of epilepsy.

关 键 词：痫性发作 谷氨酸 Γ-氨基丁酸 海马 高效液相色谱法 

分 类 号：R742.1[医药卫生—神经病学与精神病学]