切除修复交叉互补酶1等常见耐药相关蛋白在伴神经侵犯胃癌中的表达  

Expression of chemotherapy resistance associated proteins including excision repair crosscomplementation group 1 in gastric cancer patients with perineural invasion

作　　者：戴东方[1] 陈德玉[1] 李小琴[1] 顾汉刚[1] 陶清 王德强[1] Dai Dongfang;Chen Deyu;Li Xiaoqin;Gu Hangang;Tao Qing;Wang Deqiang(Department of Oncology,the Affiliated Hospital of Jiangsu University,Zhenjiang 212001,China)

机构地区：[1]江苏大学附属医院肿瘤科,江苏镇江212001

出　　处：《南京医科大学学报（自然科学版）》2019年第11期1584-1588,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助项目(81301765);江苏省青年医学重点人才项目(QNRC2016829);镇江市科技计划项目(SH2015054,SH2016038);江苏大学附属医院“5123”人才计划(51232017301)。

摘　　要：目的:检测切除修复交叉互补酶1(excision repair cross-complementation group 1,ERCC1)等常见耐药相关蛋白在伴神经侵犯胃癌患者中的表达。方法:回顾性分析242例胃腺癌患者的临床资料,免疫组化染色检测肿瘤组织中ERCC1、微管蛋白β3、胸苷酸合酶(thymidylate synthase,TS)、DNA拓扑异构酶2(topoisomerase 2-alpha,TOP2A)及P糖蛋白的蛋白表达水平,比较有和无神经侵犯者上述分子标志物表达的差异。结果:108例(44.6%)有神经侵犯,其肿瘤最大径≥5 cm、低分化、伴肿瘤脉管侵犯及TNM分期Ⅲ~Ⅳ期的比例均显著高于无神经侵犯者(P <0.05)。伴神经侵犯者的ERCC1高表达率显著高于无神经侵犯者(63.9%vs. 46.5%,P <0.05),而其他耐药相关蛋白的高表达率在两组间均无统计学差异(P> 0.05)。亚组分析显示,有和无神经侵犯者之间ERCC1高表达率的差异主要出现于男性(64.8%vs. 43.1%,P=0.026)和肿瘤低分化的患者中(76.3%vs.42.9%,P=0.006)。结论:伴神经侵犯胃癌患者的ERCC1蛋白表达水平升高,提示了神经侵犯影响化疗疗效的可能原因。Objective:To investigate the expressions of proteins associated with chemotherapy resistance including excision repair cross-complementation group 1(ERCC1)in gastric cancer patients perineural invasion(PNI). Methods:A retrospective study was conducted in 242 patients with gastric adenocarcinoma. The expressions of tubulin β3,thymidylate synthase(TS),P-glycoprotein,topoisomerase 2-alpha(TOP2 A)and ERCC1 were detected by immunohistochemistry in paraffin-embedded tumor tissues and were compared between patients with and without PNI. Results:PNI was detected in 108 patients(44.6%),and the proportions of patients with the longest diameter of tumor ≥5 cm,poor histological differentiation,lymphovascular invasion and TNM stages of Ⅲ~Ⅳ were significantly higher in these patients than those in patients without PNI(P < 0.05). The high expression rate of ERCC1 in patients with PNI was significantly higher than those in those without PNI(63.9% vs. 46.5%,P < 0.05),and this difference was mainly found in subgroups of male(64.8% vs. 43.1%,P=0.026)and poor differentiation(76.3% vs. 42.9%,P=0.006). The high expression rates of tubulin β3,TS,P-glycoprotein and TOP2 A were not significantly different between these two groups(P > 0.05). Conclusion:Gastric cancer patients with PNI had an increased expression level of ERCC1 protein,indicating the possible mechanism of poor chemotherapy effect induced by PNI.

关 键 词：胃癌 神经侵犯 切除修复交叉互补酶1 

分 类 号：R735.2[医药卫生—肿瘤]