钳夹压力法建立大鼠坐骨神经损伤模型的研究  

Establishment of sciatic nerve injury model of rat by clamp pressure

作　　者：王启文 宋春莉[2] 沈梅[1,3] 叶冬梅 李莎[1] 丛林 WANG Qiwen;SONG Chunli;SHEN Mei(Affiliated Zhongshan Hospital of Dalian University,Liaoning,Dalian,116001)

机构地区：[1]大连大学附属中山医院,辽宁省大连市116001 [2]大连医科大学附属第一医院,辽宁省大连市116001 [3]深圳市龙华区人民医院

出　　处：《中国康复医学杂志》2020年第2期135-140,共6页Chinese Journal of Rehabilitation Medicine

基　　金：大连市医学科学研究计划项目(1711095);国家自然科学基金青年基金项目(81601982)

摘　　要：目的:建立钳夹压力可量化的坐骨神经损伤模型。方法:将30只SD大鼠随机分成5组,每组6只,根据不同钳夹压力将大鼠分为1N组、2N组、4N组、8N组和16N组。手术暴露坐骨神经,采用止血钳钳夹大鼠的坐骨神经,同时用薄膜型压力传感器测量钳夹压力。用肌电图诱发电位仪分别测量钳夹前及钳夹30min后大鼠的坐骨神经复合肌肉动作电位(CMAP),并测定钳夹后第2天各压力组大鼠的坐骨神经功能指数(SFI),并观察神经纤维组织HE染色变化。结果:压力1N组、2N组、4N组、8N组和16N组钳夹后与钳夹前的坐骨神经CMAP比较,其潜伏期均明显延长(P<0.05),MNCV均显著下降(P<0.01),MNCV下降值分别占各自正常对照组的25%、51%、69%、67%、94%;各压力组近端波幅均显著下降(P<0.05),分别下降16%、34%、68%、74%、89%。各压力组SFI均显著增大(P<0.01)。组织形态学观察表明神经纤维组织随压力增高出现形态改变。结论:钳夹压力法建立大鼠坐骨神经1N钳夹压力造成坐骨神经中度损伤模型,压力2-16N造成重度损伤模型。Objective: To explore a model of sciatic nerve injury of rat with quantifiable clamp pressure.Method: Thirty SD rats were randomly divided into 5 groups(1 N, 2 N, 4 N, 8 N and 16 N), 6 in each group.The sciatic nerve was exposed surgically and clamped by hemostat. The pressure of the clamp was measured by a membrane type pressure sensor. Before and 30 minutes after clamp, the composite muscle action potential(CMAP) was recorded. The amplitude and latency of CMAP and motor nerve conduction velocity(MNCV)were measured. The sciatic nerve function index(SFI) was measured on the 2 nd day after clamping. Histological study on sciatic nerve injure was also performed.Result: There was significant delay in the latency(P<0.05) and decrease in the motor nerve conduction velocity in the 1 N, 2 N, 4 N, 8 N and 16 N group after the clamp(P<0.01), which decreased by 25% 、51% 、69% 、67% 、94%, respectively. The proximal amplitude of CMAP after clamp was also decreased dramatically in each pressure group(P<0.05), and the decline values accounted for 16% 、34% 、68% 、74% 、89%. The SFI in each pressure group increased in different degree(P<0.01). Histomorphological results showed that the greater the pressure, the more disordered the arrangement of nerve fibers.Conclusion: The clamp pressure may be a promising method for establishing a model of sciatic nerve injury of rat. The 1 N clamp force causes a moderate injury, and a severe injury model is caused by a pressure of 2-16 N.

关 键 词：坐骨神经损伤 薄膜型压力传感器 钳夹压力 运动神经传导速度 坐骨神经功能指数 

分 类 号：R745[医药卫生—神经病学与精神病学] R49[医药卫生—临床医学]