黑果枸杞LrANS基因的克隆及表达分析  

Cloning and Expression Analysis of LrANS from Lycium ruthenicum Murr

作　　者：王联星 马得森 史国民 高思丹 郭佳磊 赵伟民 李凤珍 何涛 WANG Lianxing;MA Desen;SHI Guomin;GAO Sidan;GUO Jialei;ZHAO Weimin;LI Fengzhen;HE Tao(School of Ecol-Environmental Engineering,Xining 810016,China;Key Laboratory of Landscape Plants of Qinghai Province,Xining 810016,China;State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学生态环境工程学院,西宁810016 [2]青海省园林植物与观赏园艺重点实验室,西宁810016 [3]省部共建三江源生态与高原农牧业国家重点实验室,西宁810016

出　　处：《中国细胞生物学学报》2019年第10期1929-1937,共9页Chinese Journal of Cell Biology

基　　金：国家自然科学基金项目(批准号:31660217)资助的课题~~

摘　　要：以黑果枸杞为材料,利用同源克隆和RACE技术克隆了花青素合成相关基因LrANS(Gen Bank登录号为MK713977)。序列分析表明, LrANS基因cDNA全长为1 527 bp,包含1 290 bp的开放阅读框,编码429个氨基酸;亚细胞定位显示, LrANS主要分布于细胞质及细胞膜上;同源序列比对表明, LrANS与烟草Nt ANS的氨基酸序列相似性较高,达到77.2%。RT-PCR分析显示, LrANS基因在根、茎、叶、花、青果、紫果和黑果中均有表达,且在黑果中的表达显著高于其他组织;在紫外胁迫下, LrANS基因的表达显著下降。推测LrANS基因在花青素合成中发挥着重要作用。Taking the Lycium ruthenicum Murr as materal, an anthocyanidin synthesis-related gene named LrANS(GenBank accession number MK713977) was cloned by homologous cloning and RACE methods. The sequence analysis showed that the length of LrANS gene was 1 527 bp, which contained a 1 290 bp ORF encoding 429 amino acids. Subcellular localization assays showed that the LrANS protein was localized in the cytoplasm and cytomembrane. Comparison of homology amino acid sequence indicated that LrANS had higher similarity with Nicotiana tabacum ANS(77.2%). The RT-PCR analysis showed that the LrANS gene was expressed in roots, stems, leaves, flowers, green fruits, purple fruits and black fruits, and the expression level in black fruits was significantly higher than other tissues. The LrANS gene expression was dramatically decreased under UV irradiation. It is speculated that the LrANS gene may play an important role in the anthocyanidin synthesis of L. ruthenicum.

关 键 词：黑果枸杞 花青素 LrANS基因 紫外胁迫 

分 类 号：S567.19[农业科学—中草药栽培]