北京地区不同分娩方式婴幼儿肠道菌群分析  

Analysis of intestinal flora in infants with different delivery modes in Beijing

作　　者：安云鹤[1] 安涛 武会娟[1] 程小艳[1] 马凯[1] 陈尔凝[1] 高丽娟[1] AN Yun-he;AN Tao;WU Hui-juan;CHENG Xiao-yan;MA Kai;CHEN Er-ning;GAO Li-juan(Beijing Center for Physical and Chemical Analysis,Beijing Engineering Technique Research Center for Gene Sequencing and Function Analysis,Beijing Engineering Technique Research Center for Food Safety Analysis,Beijing 100089;Beijing Taihe Maternity Hospital,Beijing 100195,China)

机构地区：[1]北京市理化分析测试中心北京市基因测序与功能分析工程技术研究中心北京市食品安全分析测试工程技术研究中心,北京100089 [2]北京太和妇产医院,北京100195

出　　处：《生物学杂志》2020年第1期45-49,共5页Journal of Biology

基　　金：北京市科学技术研究院创新团队计划课题(IG201606C1)

摘　　要：婴幼儿时期肠道菌群的定殖是肠道菌群形成的关键时期,影响未来生长发育和健康状况。旨在探讨不同分娩方式婴幼儿肠道菌群多样性差异。选取258例0~3岁北京地区健康婴幼儿肠道粪便样品,其中包括97例剖宫产样品、161例自然分娩样品。通过高通量测序技术分析表明,某些细菌的相对丰度发生显著改变。与剖宫产婴幼儿相比,自然分娩婴幼儿肠道中的放线菌门(优势菌,13.92%VS 16.86%)和酸杆菌门(劣势菌,0%VS 0.00204%)的相对丰度显著升高(P<0.05);而厚壁菌门(优势菌,67.17%VS 61.16%)和柔膜菌门(劣势菌,0.074%VS 0.051%)的相对丰度明显下降(P<0.05)。在属水平上,优势菌中的双歧杆菌属(13.01%VS 15.74%)在自然分娩婴幼儿肠道粪便中的含量显著升高,而梭菌属(2.25%VS 1.53%)显著降低。Intestinal microflora plays an important role in the whole life of human beings and the colonization of intestinal microflora in infants is critical for the formation of intestinal flora,which will affect their future growth,development and health.The purpose of this study is to explore the composition changes of intestinal microflora caused by delivery mode and its effects on the growth and development of 0-3 years old infants.In this study,258 cases of intestinal fecal samples from healthy infants of 0-3 years old in Beijing were collected,including 97 caesarean section samples and 161 natural childbirth samples.The results by high-throughput sequencing technology showed that the relative abundance of some bacteria changed significantly.Compared with cesarean section group,the relative abundance of Actinobacteria(dominant bacteria,13.92%VS 16.86%)and Acidobacteria(inferior bacteria,0%VS 0.00204%)in the intestine of natural childbirth infants increased significantly(P<0.05);and the relative abundance of Firmicutes(dominant bacteria,67.17%VS 61.16%)and Tenericutes(inferior bacteria,0.074%VS 0.051%)decreased significantly(P<0.05).At the genera level,the content of Bifidobacterium(13.01%VS 15.74%)in the dominant bacteria increased significantly in the intestinal feces of the natural childbearing infants,while Clostridium(2.25%VS 1.53%)decreased significantly.

关 键 词：分娩方式 婴幼儿 肠道菌群 年龄 BMI 

分 类 号：R446.5[医药卫生—诊断学]