海带多糖抑制平滑肌细胞增殖及抗氧化活性研究  

Inhibitory Effects of Laminaria Japonica Polysaccharides on Proliferation and Antioxidant Activities of Vascular Smooth Muscle Cells

作　　者：彭臻菲[1] 李泳宁[1] 魏碧娜 林碧珠 PENG Zhen-fei;LI Yong-ning;WEI Bi-na;LIN Bi-zhu(Fujian Health College,Fuzhou,Fujian 350101,China)

机构地区：[1]福建卫生职业技术学院

出　　处：《福建农业学报》2019年第12期1457-1462,共6页Fujian Journal of Agricultural Sciences

基　　金：福建省医学创新基金项目（2015-CXB-27）;福建卫生职业技术学院科研基金项目（2014-1-2）

摘　　要：【目的】研究海带多糖对H2O2诱导血管平滑肌细胞(vascular smooth muscle cells,VSMC)增殖及胞内脂质过氧化物生成量的影响,为阐明海带多糖对VSMC的作用机制奠定基础。【方法】以H2O2为诱导剂建立体外VSMC增殖模型,通过四甲基偶氮唑盐法和细胞形态观察评价海带多糖对H2O2诱导VSMC生长和增殖的影响,同时以丙二醛为指标考察海带多糖对H2O2诱导VSMC胞内脂质过氧化物生成量的影响。【结果】以50μmol·L−1 H2O2为诱导剂建立VSMC体外增殖模型。四甲基偶氮唑盐法测定结果表明海带多糖对H2O2诱导VSMC增殖具有显著抑制活性,最大增殖抑制率达到73.56%。细胞形态学观察结果表明海带多糖作用下H2O2诱导的VSMC生长状态发生改变且细胞数量显著减少。丙二醛检测结果表明海带多糖作用下VSMC胞内脂质过氧化物量显著减少。【结论】海带多糖能够抑制H2O2诱导的VSMC增殖,且显著降低VSMC胞内脂质过氧化物生成量。【Objective】Effects of Laminaria japonicapolysaccharides on the proliferation and lipid peroxide production of H2O2-induced vascular smooth muscle cells(VSMCs)were examined in vitro for further understanding on the metabolic mechanism.【Method】Proliferation of VSMCs was artificially induced by using 50μmol·L−1 H2O2.Inhibitory rate on the proliferation by the L.japonica polysaccharides were determined by a methyl thiazolyl tetrazolium(MTT)assay.Morphological changes on VSMCs were observed under a microscope.Malondialdehyde(MDA)was employed as an indicator of the lipid peroxide production in VSMCs.【Result】A significant inhibition effect of the polysaccharides on the H2O2-induced VSMC proliferation with the greatest rate of 73.56%was shown by MTT.Morphologically,the number of VSMCs significantly decreased after an exposure to the polysaccharide.The MDA analysis indicated that the lipid peroxide production by VSMCs significantly declined after the treatment with L.japonica polysaccharides.【Conclusion】The L.japonica polysaccharides significantly inhibited the proliferation and lipid peroxide production in the H2O2-induced VSMCs.

关 键 词：海带多糖 血管平滑肌细胞 H2O2诱导 细胞增殖 脂质过氧化物 

分 类 号：P745[天文地球—海洋生物学]