分割照射对肝癌移植瘤小鼠脾脏免疫细胞的影响  

Effect of fractionated radiotherapy of transplanted hepatocellular carcinoma on the splenic immune cells in mice

作　　者：姚丹 张俊玲 郑旺 陈倩萍 胡松玲 潘燕[1] 邵春林[1] Yao Dan;Zhang Junling;Zheng Wang;Chen Qianping;Hu Songling;Pan Yan;Shao Chunlin(Institute of Radiation Medicine,Fudan University,Shanghai 200032,China)

机构地区：[1]复旦大学放射医学研究所,上海200032

出　　处：《中华放射医学与防护杂志》2020年第2期82-87,共6页Chinese Journal of Radiological Medicine and Protection

基　　金：国家重点研发计划项目(2017YFC0108604);国家自然科学基金(31770910,31570850)。

摘　　要：目的探讨肝癌皮下移植瘤小鼠的分割照射对远端脾脏组织的影响。方法构建荷瘤C57BL/6 J小鼠模型,取对数期生长的小鼠肝癌细胞Hepa 1-6接种于小鼠右侧皮下(1×10^7/只),荷瘤小鼠按随机数表法分为荷瘤对照组和照射组,每组20只。另设10只健康小鼠作为健康对照组。照射组皮下肿瘤给予8 Gy×3次X射线局部照射,剂量率为0.883 Gy/min。照射后第7、14天检测小鼠肿瘤脏器指数、脾脏脏器指数、脾脏病理学改变,以及脾脏T淋巴细胞亚群、B淋巴细胞亚群、NK细胞的变化。结果与荷瘤对照组相比,照后7、14 d,照射组小鼠肿瘤脏器指数显著降低(t=4.649、26.34,P<0.05),脾脏中NK细胞显著升高(t=3.952、3.633,P<0.05),CD3+、CD4+、CD3+CD4+淋巴细胞以及CD4+/CD8+比值在照后7 d显著降低(t=3.193、3.656、3.219、2.641,P<0.05),CD3+淋巴细胞在照后14 d显著降低(t=3.031,P<0.05),其余指标脾脏脏器指数、B淋巴细胞、CD3+CD4+淋巴细胞、CD8+淋巴细胞变化不显著。结论肿瘤局部照射可引起远隔器官内淋巴细胞比例失衡,导致机体免疫力下降,为放疗免疫损伤提供了新的诠释。Objective To investigate the effect of fractionated radiotherapy on the immune system of mice with subcutaneously transplanted hepatocellular carcinoma.Methods Logarithmic growth of mouse hepatocellular carcinoma cells Hepa 1-6 were inoculated subcutaneously on the right side of C57BL/6 J mice(1×10^7 cells/mice).The tumor-bearing mice were randomly divided into control group(Ctrl)and irradiation group(IR),20 mice in each group.Additionally,10 healthy mice were set as normal control group.Local fractionated X-ray irradiation of 8 Gy×3 fraction was given to the subcutaneous tumors,and the dose rate was 0.883 Gy/min.At 7 and 14 d after irradiation,the tumor organ index,spleen organ index,spleen pathological changes,and splenic T lymphocyte subsets,B lymphocyte subsets,and NK cells were detected.Results Compared with Ctrl,at 7 and 14 d after irradiation,the tumor organ index decreased(t=4.649,26.34,P<0.05),and the percentage of NK cells increased significantly(t=3.952,3.633,P<0.05).The percentages of CD3+,CD4+,CD3+CD4+lymphocytes and the ratio of CD4+/CD8+lymphocyte decreased at 7 d after irradiation(t=3.193,3.656,3.219,2.641,P<0.05),and the percentage of CD3+lymphocyte decreased at 14 d after irradiation(t=3.031,P<0.05).But after irradiation,there were no significant changes in spleen organ index,B lymphocyte,CD3+CD4+lymphocyte and CD8+lymphocyte.Conclusions Local hepatoma radiotherapy causes imbalance of lymphocytes in distal spleen of mice and hence reduces immunity,which provides a novel mechanism of radiological immunity damage.

关 键 词：肝癌 分割照射 脾脏淋巴细胞 

分 类 号：R735[医药卫生—肿瘤]