Decreased morphogenetic potential in peach palm stem-like cells in long-term in vitro conditions  

作　　者：Erika Mendes Graner Gilvano Ebling Brondani Cristina Vieira de Almeida Katherine Derlene Batagin-Piotto Marcilio de Almeida 

机构地区：[1]Luiz de Queiroz College of Agriculture,University of Sao Paulo,Piracicaba,SP,Brazil [2]Department of Forest Sciences,Campus Universitario,Federal University of Lavras,Lavras,MG,Brazil [3]In Vitro Palm(Consulting,Study and Biological Development Ltda.),Piracicaba,SP,Brazil

出　　处：《Journal of Forestry Research》2020年第2期485-495,共11页林业研究（英文版）

摘　　要：Peach palm(Bactris gasipaes Kunth)has been micropropagated from pre-procambial cells that provide stem-like cell niches,(i.e.,pre-procambial cells),multipotent,pluripotent and totipotent for direct vascularization,adventitious buds and somatic embryogenesis,respectively.The direct induction of adventitious buds and somatic embryogenesis reduces the frequency of mutations when compared to indirect morphogenesis.Long-term in vitro cultivation of perennial species such as peach palm cause the clones to age and deteriorate;however,the consequences for morphogenesis potential are not fully clear.The morphogenic potential of peach palm clones established and in vitro cultivated for 8 years(regeneration of adventitious buds without callus formation)was investigated in leaves,roots and stem bases using histological and histochemical analyses.Data from long-term cultures(8-years-old)was compared to data from short-term cultures(1-year-old).Morphogenic pathways monitoring for direct induction of somatic embryos and adventitious buds revealed a strong morphogenic reduction potential in the pre-procambial cells,parenchyma cells in the proximal region of stem bases,and external cells of leaf sheaths.Initial cells of shoot apical meristems and pre-procambial cells commit cell reprogramming to the undifferentiated state and subsequent acquisition of cellular competence.These results are applicable in the micropropagation of peach palm,with consideration to obtaining clones and their long-term in vitro culture.

关 键 词：MICROPROPAGATION MULTIPOTENCY Pluripotency TOTIPOTENCY Pre-procambial cells Peach palm Bactris gasipaes 

分 类 号：Q942[生物学—植物学]