TLR5基因多态性与非贲门胃癌的关联研究  

An association study between polymorphisms in the TLR5 gene and risk of non-cardia gastric cancer

作　　者：高芳[1] 卫星如 马立聪 田旭阳[3] 党彤[3] 贾彦彬[2,3,4] Gao Fang;Wei Xingru;Ma Licong(Dept of Medical Technology,Baotou Medical College,Baotou 014060;Dept of Basic Medicine and Forensic Medicine,Baotou Medical College,Baotou 014060;Inner Mongolia Institute of Digestive Diseases,The Second Affiliated Hospital of Baotou Medical College,Baotou 014030)

机构地区：[1]包头医学院医学技术与麻醉学院,包头014060 [2]包头医学院基础医学与法医学院,包头014060 [3]包头医学院第二附属医院内蒙古消化病研究所,包头014030 [4]包头医学院护理学院,包头014060

出　　处：《安徽医科大学学报》2020年第1期114-117,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30960169、81250024、81650017);内蒙古自然科学基金[编号:2016MS0805、2017MS(LH)0807];包头医学院科学研究基金项目(编号:BYJJ-DF 201603)

摘　　要：目的探讨TLR5基因单核苷酸多态性与非贲门胃癌发病风险的关系,为非贲门胃癌的早期诊断和治疗提供理论基础和实验依据。方法用病例对照关联研究的方法,在包头地区汉族人群中收集了288例经组织病理学诊断为非贲门胃癌的患者和281例正常对照。用Taq Man法对TLR5基因SNP rs34270714、rs5744174、rs851139、rs17163737进行基因分型;Haploview 4.0软件被用来构建单体型,同时运用非条件性Logistic回归计算比值比(OR)及其95%可信区间(CI),评估各等位基因、基因型及单体型与非贲门胃癌发病风险的关系。结果包头地区汉族人群中TLR5基因SNP rs17163737与非贲门胃癌的发病风险相关联,与携带GG基因型的人群比较,GT基因型携带者非贲门胃癌的发病风险提高1.605倍(GT vs GG:OR=1.605,95%CI=1.131~2.280),其余SNP与非贲门胃癌的发病风险无关联(P>0.05)。同时,单体型分析结果显示:rs5744174-rs851139-rs17163737存在强连锁不平衡,构成单体型区域(Block);然而各单体型均与非贲门胃癌发病风险无关联。结论包头汉族人群中TLR5基因SNP rs17163737 GT基因型携带者非贲门胃癌的发病风险增高。Objective To investigate the association between single nucleotide polymorphisms(SNP)of the TLR5 gene and the risk of non-cardia gastric cancer,and to supply theoretical and experimental basis for early diagnosis and treatment of non-cardia gastric cancer.Methods Using case-control study design,288 non-cardia gastric cancer patients diagnosed by histopathology were selected as the case group while 281 community physical examinees were selected as the normal control group,and they were all Han population in Baotou.SNP rs34270714,rs5744174,rs851139 and rs17163737 were genotyped using TaqMan method.Haplotype was constructed by Haploview software 4.0.Odds ratios(OR)and 95%confidence intervals(CI)were measured by unconditional Logistic regression analysis to evaluate the correlations of the alleles,genotypes and haplotypes to the susceptibility to non-cardia gastric cancer.Results In Baotou Han population,the SNP rs17163737 of TLR5 was associated with the risk of non-cardia gastric cancer.Compared to GG genotype,the GT genotype was associated with a significantly increased risk of non-cardia gastric cancer(GT vs GG:OR=1.605,95%CI=1.131~2.280).Other SNPs were not associated with the risk of non-cardia gastric cancer(P>0.05).In addition,there was a strong linkage disequilibrium between rs5744174,rs851139 and rs17163737,which constituted a haplotype region(Block).However,there was no association between any haplotypes and the risk of non-cardia gastric cancer.Conclusion The carriers of TLR5 rs17163737 GT genotype have an increased risk of non-cardia gastric cancer in Baotou Han population.

关 键 词：TLR5 基因多态性 非贲门胃癌 

分 类 号：R394.5[医药卫生—医学遗传学]