二烯丙基二硫下调TGF-β1/Rac1通路抑制人胃癌细胞β-catenin表达  

DADS inhibitsβ-catenin expression by down-regulating TGF-β1/Rac1 pathway in human gastric cancer MGC803 cells

作　　者：苏波[1,2] 丁恩 曾颖[2] 夏红[2] 刘芳[2] 苏琦[2] SU Bo;DING En;ZENG Ying;XIA Hong;LIU Fang;SU Qi(Institute of Pharmacy and Pharmacology,Key Lab for Pharmacoproteomics of Hunan Provincial University,University of South China,Hengyang Hunan 421001,China;Cancer Research Institute,Center for Gastric Cancer Research of Hunan Province,Key Lab of Cancer Cellular and Molecular Pathology of Hunan Provincial University,University of South China,Hengyang Hunan 421001,China;Dept of Oncology,the Affiliated Hospital,Hunan College of Traditional Chinese Medicine,Zhuzhou Hunan 412012,China)

机构地区：[1]南华大学药学与药理学研究所,湖南省高校药物蛋白质组学重点实验室,湖南衡阳421001 [2]南华大学肿瘤研究所,湖南省胃癌研究中心,湖南省高校肿瘤细胞与分子病理学重点实验室,湖南衡阳421001 [3]湖南省中医药专科学校附属医院肿瘤科,湖南株洲412012

出　　处：《中国药理学通报》2020年第3期393-398,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金(81641112,81374013)。

摘　　要：目的研究二烯丙基二硫(diallyl disulfide,DADS)是否通过下调TGF-β1/Rac1通路抑制人胃癌MGC803细胞β-catenin表达。方法实验组分为6组,对照组(Control),DADS处理组,TGF-β1处理组,TGF-β1+SB431542组,TGF-β1+DADS组,TGF-β1+NSC23766组。RT-PCR、Western blot分别检测TGF-β1、Rac1、β-catenin、E-cadherin和vimentin mRNA和蛋白水平。结果DADS下调TGF-β1、Rac1、β-catenin和vimentin,上调E-cadherin表达。TGF-β1受体阻断剂SB431542和Rac1抑制剂NSC23766下调β-catenin和vi-mentin,上调E-cadherin表达。结论DADS通过下调TGF-β1/Rac1通路抑制人胃癌MGC803细胞β-catenin表达。Aim To investigate whether diallyl disul-fide(DADS)inhibitsβ-catenin expression by down-regulating the TGF-β1/Rac1 pathway in human gastric cancer MGC803 cells.Methods The experimental group was divided into six groups,control,DADS,TGF-β1,TGF-β1+SB431542,TGF-β1+DADS,and TGF-β1+NSC23766 group.RT-PCR and Western blot were performed to detect mRNA and protein lev-els,respectively.Results DADS down-regulated TGF-β1,Rac1,β-catenin and vimentin,and up-regu-lated E-cadherin expression.TGF-β1 receptor blocker SB431542 and Rac1 inhibitor NSC23766 down-regula-tedβ-catenin and vimentin,up-regulated E-cadherin expression.Conclusions DADS inhibitsβ-catenin ex-pression by down-regulating the TGF-β1/Rac1 pathway in human gastric cancer MGC803 cells.

关 键 词：二烯丙基二硫 转化生长因子Β1 RAC1 Β-CATENIN 人胃癌细胞 上皮细胞向间质细胞转化 

分 类 号：R735.2[医药卫生—肿瘤]