常压室温等离子体诱变选育高产核黄素枯草芽孢杆菌  

Screening of high-yield riboflavin Bacillus subtilis strain by atmospheric and room temperature plasma

作　　者：郭佳欣 张培基 刘丁玉[1,2,3,4] 洪坤强 陈涛 王智文[1,2,3,4] GUO Jiaxin;ZHANG Peiji;LIU Dingyu;HONG Kunqiang;CHEN Tao;WANG Zhiwen(School of Chemical Engineering and Technology,Tianjin University,Tianjin 300350,China;Key Laboratory of Systems Bioengineering(Ministry of Education),Tianjin University,Tianjin 300350,China;Frontier Science Center for Synthetic Biology,Tianjin University,Tianjin 300350,China;SynBio Research Platform,Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300350,China)

机构地区：[1]天津大学化工学院,天津300350 [2]系统生物工程教育部重点实验室,天津300350 [3]合成生物学前沿科学中心,天津300350 [4]天津化学化工协同创新中心合成生物学平台,天津300350

出　　处：《食品与发酵工业》2020年第4期28-33,共6页Food and Fermentation Industries

基　　金：国家自然科学基金(21576200; 21776209; 21621004)

摘　　要：该研究以BS120作为出发菌株,通过常压室温等离子体诱变(atmospheric and room temperature plasma,ARTP)技术进行诱变处理,第一轮以40 mg/L 8-氮鸟嘌呤为筛选拮抗物进行筛选,得到核黄素产量和得率分别提升61.60%和58.12%的菌株BSG1。第二轮诱变以300 mg/L寡霉素为筛选拮抗物进行筛选,筛选获得菌株BSG3,核黄素产量和得率较BS120分别提升83.59%和78.76%。将核黄素操纵子表达质粒pMX45转入BSG3中,得到菌株BSG5,核黄素产量达到(4467.08±99.47)mg/L,得率为(42.56±1.25)mg/g葡萄糖,较BS120分别提高140.94%和120.52%,展现了良好的核黄素发酵性能和遗传稳定性。Bacillus subtilis is an important riboflavin producing strain in microbial fermentation.It is significant importance to breed high-yield-riboflavin Bacillus subtilis strains in industrial production.In this work,the parent riboflavin-producing strain BS120 was treated with atmospheric and room temperature plasma(ARTP)for the following screening.In the first round,the production and yield of riboflavin for mutant strain BSG1 screened with 40 mg/L 8-azaguanine increased by 61.60%and58.12%,respectively.In the second round,the production and yield of riboflavin for mutant strain BSG3 screened with 300 mg/L oligomycin increased by 83.59%and 78.76%compared with BS120,respectively.Riboflavin operon expression plasmid pMX45 was transformed into BSG3 to generate BSG5 in order to further increase the riboflavin production.The results showed that riboflavin production of BSG5 reached(4467.08±99.47)mg/L,and the yield was(42.56±1.25)mg/g glucose,which was 140.94%and 120.52%higher than that of BS120 respectively.These mutant strains showed excellent riboflavin fermentation performance and genetic stability during the test.

关 键 词：枯草芽孢杆菌 核黄素 常压室温等离子体诱变 诱变选育 8-氮鸟嘌呤 寡霉素 

分 类 号：TQ924[轻工技术与工程—发酵工程]