口腔鳞癌中NF-κBp65、IL-6表达与巨噬细胞极化的关系  

The relationship among NF-κB p65,IL-6 and macrophage polarization in oral squamous cell carcinoma

作　　者：钱娴娴 冯红超 宋宇峰[3] QIAN Xianxian;FENG Hongchao;SONG Yufeng(School of Stomatology,Guizhou Medical University,China,550004;Guiyang Municipal Stomatological Hospital;Departmnent of Food and Drug Administration Bureau in Guizhou Province,Guiyang)

机构地区：[1]贵州医科大学口腔医学院,贵阳550004 [2]贵阳市口腔医院,贵阳550004 [3]贵州省食品药品监督管理局

出　　处：《实用口腔医学杂志》2020年第1期10-14,共5页Journal of Practical Stomatology

基　　金：贵阳市科技计划项目[编号:筑科合同(2018)1-56号];贵阳市卫生和计划生育委员会科学技术项目[编号:(2016)筑卫计科技合同字第026号]。

摘　　要：目的:探讨口腔鳞状细胞癌(OSCC)中核因子-κBp65(NF-κBp65)、白介素-6(IL-6)的表达及其二者与巨噬细胞极化之间的关系。方法:采用免疫组织化学SP法检测在15例正常组织及42例OSCC组织中NF-κBp65、IL-6的表达及CD68^+、CD163^+巨噬细胞的浸润情况,并结合临床病理参数进行对比分析。结果:在OSCC中,NF-κBp65、IL-6的表达及CD68^+、CD163^+巨噬细胞的浸润数量均高于正常组织(P<0.05);NF-κBp65的表达、CD68^+及CD163^+巨噬细胞的浸润数量与淋巴结转移有关(P<0.05);NF-κBp65与IL-6、CD68^+、CD163^+巨噬细胞在OSCC中的表达或浸润数量均呈正相关(P<0.05);CD68^+、CD163^+巨噬细胞的浸润数量呈正相关(P<0.05)。结论:在OSCC中,NF-κBp65和IL-6的表达增高并参与了巨噬细胞极化过程,三者与肿瘤的侵袭与转移密切相关,可能在OSCC发展中存在相互促进作用。Objective:To investigate the relationship among NF-κB p65,IL-6 and macrophage polarization in oral squamous cell carcinoma(OSCC).Methods:The immunohistochemical technique with SP method was used to detect the expression of NF-κBp65,IL-6,CD68+macrophages and CD163+macrophages in 42 cases of OSCC tissues and 15 normal oral mucosa tissues.The correlation of the indicators with the clinicopathological data was analyzed.Results:In the OSCC group,the expression of NF-κBp65,IL-6,CD68+macrophages and CD163+macrophages was significantly higher than that of the normal tissue group(P<0.05).The expression of NF-κBp65,CD68+and CD163+macrophages was correlated with lymph node metastasis(P<0.05).In OSCC there was a positive correlation between the expression of NF-κBp65 and IL-6(P<0.05),between the expression of NF-κBp65 and CD68+macrophages(P<0.05),between the expression of NF-κBp65 and CD163+macrophages(P<0.05),between expression of CD68+macrophages and CD163+macrophages in OSCC(P<0.05).Conclusion:In OSCC,the expression of NF-κBp65 and IL-6 is increased,the polarization of macrophages is promoted,the 3 indicators are closely related to tumor invasion and metastasis,and may promote each other in the development of OSCC.

关 键 词：口腔鳞状细胞癌(OSCC) NF-ΚB IL-6 CD68 CD163 巨噬细胞 

分 类 号：R739.8[医药卫生—肿瘤]