培养基加氟后渗透压变化对成釉细胞超微结构影响的观察  

The change of osmotic pressure in the culture medium with additional fluoride and its influence on ameloblast ultrastructure

作　　者：张乐[1] 王垚 王军强[1] 曹伟靖[1] 张学武 ZHANG Le;WANG Yao;WANG Junqiang;CAO Weijing;ZHANG Xuewu(Affiliated Hospital of Yan'an University,China,716000;Yan'an Traditonal Medicine Hospital)

机构地区：[1]延安大学附属医院,716000 [2]延安市中医医院

出　　处：《实用口腔医学杂志》2020年第1期41-45,共5页Journal of Practical Stomatology

摘　　要：目的:观察含有不同浓度氟化钠、氯化钠和甘油的DMEM培养基内成釉细胞超微结构的变化。方法:将体外培养5 d的成釉细胞,分别换含不同浓度氟化钠、氯化钠、甘油的DMEM培养基中(渗透压为324.0~330.8 Pa),培养48 h后在透射电镜下观察各组成釉细胞超微结构变化情况。结果:透射电子显微镜下观察:对照组细胞核周及细胞器结构正常;42 mg/L氟化钠(渗透压324.5 Pa)组细胞大多体积变小,细胞核皱缩,高尔基体水肿,部分粗面内质网扩张,张力原纤维稀疏;84 mg/L氟化钠(渗透压330.8 Pa)组细胞膜溶解破坏,细胞核溶解,胞浆,细胞器溢出,内质网疏松形成空泡,线粒体嵴消失,张力原纤维溶解模糊,染色体出现凝集。其余各实验组(渗透压324.0~330.1 Pa)与对照组超微结构情况相似。结论:培养基加氟后渗透压变化在324.0~330.8 Pa之间对成釉细胞的超微结构无明显影响。Objective:To study the change of osmotic pressure(OP)in the culture medium with additional fluoride and its influence on ameloblast ultrastructure.Methods:Ameloblasts were cultured in vitro for 5 days,and the DMEM medium containing different concentrations of NaF,NaCl and glycerol with the OP of 324.0~330.8 Pa was used for the culture.After culture for 48 h,the ultrastructure changes of ameloblasts were observed under transmission electron microscope.Results:The perinuclear and organelle structures of the control group were normal.Most of the cells in 42 mg NaF(OP=324.5 Pa)group became smaller,nuclear shrinkage,rough endoplasmic reticulum dilatation,Golgi complex edema,sparse tensile fibrils were observed.In 84 mg NaF group(OP=330.8 Pa)dissolution and destruction of cell membrane,nucleolysis,cytoplasm and organelle overflow,loose endoplasmic reticulum vacuoles,mitochondrial ridge disappearance,tensioning fibril dissolve and blur were found.The ultrastructure of ameloblasts in the other experimental groups was similar to that of the control group.Conclusion:The osmotic pressure of 324.0-330.8 Pa of culture medium has no significant effect on the ultrastructure of ameloblasts.

关 键 词：渗透压(OP) 氟牙症 成釉细胞 超微结构 

分 类 号：R780.2[医药卫生—口腔医学]