基于多元统计方法和顶空-气相色谱-质谱联用技术对瓜馥木不同部位挥发性成分的比较研究  

Comparative Research on Volatile Components in Different Parts of Fissistigma oldhamii Using HS-GC-MS and Multivariate Statistical Analysis

作　　者：胡海波[1,3,4] 李耀利 程庚金生[1,3] 邓婧怡 李加林 程齐来[1,3] 黄浩 Hu Haibo;Li Yaoli;Chenggeng Jinsheng;Deng Jingyi;Li Jialin;Cheng Qilai;Huang Hao(School of Pharmacy,Gannan Medical College,Ganzhou 341000;School of Pharmacy,Peking University,Beijing 100191,China;Hakka Medical Resource Research Institute,National Engineering Research Center for Modernization of Traditional Chinese Medicine,Ganzhou 341000,China;Department of Biology,Animal Physiology and Neurobiology Section,KU Leuven,Leuven3000,Belgium)

机构地区：[1]赣南医学院药学院,赣州341000 [2]北京大学药学院,北京100191 [3]国家中药现代化工程技术研究中心客家中医药资源研究分中心,赣州341000 [4]鲁汶大学动物研究所,鲁汶300

出　　处：《世界科学技术-中医药现代化》2019年第8期1724-1730,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委员会地区基金项目（81660639）：基于质谱联用和激光显微切割技术的瓜馥木药用部位及安全性评价研究，负责人：胡海波;江西省教育厅科学技术研究项目（GJJ150941）：香藤的药用部位评价研究，负责人：胡海波

摘　　要：目的分析瓜馥木中的挥发性成分,比较根、茎、叶和虫瘿中所含挥发性成分的差异。方法采用顶空-气相色谱-质谱(HS-GC-MS)联用技术分析瓜馥木不同部位的挥发性成分,并利用XCMS-ONLINE的组学方法和多元统计方法分析处理数据,包括偏最小二乘法判别分析(PLS-DA)、正交校正的偏最小二乘分析(OPLS-DA),辅以保留指数(RI)完成各挥发性成分的定性和半定量分析。结果共定性出41种挥发性成分,其中根含24种成分,茎含38种成分,叶含35种成分,虫瘿含25种成分,各部位的共有成分19种。结论瓜馥木挥发性成分主要是烯烃类和脂肪烃衍生物类,不同部位的挥发性成分有显著差异,研究结果将为该药材的质量标准科学制定和民间使用提供依据和借鉴,并为进一步的药效评价、资源开发利用奠定基础。Objective To comparatively perform qualitative and semi-quantitative analysis on the volatile constituents inroots, stems leaves and insect gells of Fissistigma oldhamii. Methods The volatile components were analyzed by HS-GC-MS, and the obtained data were treated by XCMS-online and multivariate statistical analysis, including partial leastsquares discriminant analysis(PLS-DA) and orthogonal partial least squares discriminant analysis(OPLS-DA). Theretention index(RI) method was used to confirm the result of qualitative analysis, and the semi-quantitative relativepercentage content of each component was calculated with the relative fragment ion. strength, which values of variableimportance for the projection(VIP) are more than 1. Results There were 41 components identified from 4 parts of F.oldhamii, including 19 common components in different parts. The roots, stems, leaves and insect galls had 24, 38, 35 and 25 compounds detected separately. Conclusion The principal volatile constituents were olefins, aliphatichydrocarbons and their derivatives, which were different in 4 parts. The results would provide basis for the furtherapplication, efficacy evaluation, scientific development of quality standard and utilization of F. oldhamii.

关 键 词：瓜馥木 香藤 保留指数 气相色谱-质谱 XCMS ONLINE PLS-DA OPLS-DA 

分 类 号：R284.1[医药卫生—中药学]