miR-424对宫颈癌细胞侵袭转移能力的影响及作用机制  

Effect of mir-424 on Invasion and Metastasis of Cervical Cancer Cells and its Mechanism of Action

作　　者：韦敬锡[1] 凌永嫦 闫位娟 WEI Jing-xi;LING Yong-chang;YAN Wei-juan(Affiliated Hospital of Youjiang Medical College for Nationalities,Baise,Guangxi 533000;Institute of Radiation Protecti on and Nuclear Safety Medicine,China Center for Disease Control and Prevention,Beijing 100088)

机构地区：[1]右江民族医学院附属医院,广西百色533000 [2]中国疾病预防控制中心辐射防护与核安全医学所,北京100088

出　　处：《智慧健康》2019年第34期36-38,共3页Smart Healthcare

基　　金：百色市科学研究与技术开发计划项目,百科计20140930;广西卫生计生委医药卫生科研项目,Z2016414。

摘　　要：目的研究抑癌因子miR-424对宫颈癌细胞侵袭转移能力的影响及作用机制。方法划痕实验检测宫颈癌细胞迁移能力;ranswell小室法检测侵袭能力;WesternBlot检测处理后细胞中WNT8A、β-catenin和VEGF的蛋白表达水平;茎环法qRT-PCR检测miRNA。结果与miR-424NC组(22.45±3.25)比较,miR-424inhibitor组(3.48±0.36)细胞迁移距离降低(P<0.010);侵袭数目与miR-424NC组(165.78±12.15)比较,miR-424inhibitor组(36.54±2.35)细胞侵袭数目降低(P<0.01),VEGFmRNA表达量下调,β-catenin表达量下调。结论miR-424对肿瘤细胞迁移及增殖的作用机制可能通过抑制WNT8A表达,阻断wnt/β-catenin信号通路,进一步通过抑制VEGF表达抑制恶性肿瘤的侵袭转移。Objective To investigate the effect of tumor suppressor mir-424 on the invasion and metastasis ability of cervical cancer cells and its mechanism.Methods Cellmigration and invasion was measured by wound scratch assay and Transwell migration assay.The protein expression levels of WNT8A,beta-catenin and VEGF in the treated cells were detected by Western Blot.MiRNA was detected by stem loop qRT-PCR.Results Compared with the mir-424 NC group(22.45±3.25),the invasion number of the mir-424 inhibitor group(3.48±0.36)was decreased(P<0.010),while that of the mir-424 NC group(165.78±12.15)was decreased(P<0.01).The mRNA expression of VEGF was down-regulated,and the expression of beta-catenin was down-regulated.Conclusion The mechanism of mir-424 on the migration and proliferation of tumor cells may inhibit the WNT8A expression,block the WNT/beta-catenin signaling pathway,and further inhibit the invasion and metastasis of malignant tumors by inhibiting the expression of VEGF.

关 键 词：MiR-424 宫颈癌细胞 侵袭转移 WNT/Β-CATENIN信号通路 

分 类 号：R737.33[医药卫生—肿瘤]