离子液体超声波辅助提取白花葛茎多糖工艺优化  

Optimization of ionic liquid based ultrasonic-assisted extraction of polysaccharide from Pueraria f. niveus stem

作　　者：林志銮[1] 金晓怀 张传海[3] 马春华[3] LIN Zhi-luan;JIN Xiao-huai;ZHANG Chuan-hai;MA Chun-hua(Analysis and Testing Center,Wuyi University,Nanping 354300,China;Yushan Health Management College,Wuyi University,Nanping 354300,China;Fujian Provincial Key Laboratory of Eco-Industrial Green Technology,Wuyi University,Nanping 354300,China)

机构地区：[1]武夷学院分析测试中心,福建南平354300 [2]武夷学院玉山健康管理学院,福建南平354300 [3]福建省生态产业绿色技术重点实验室(武夷学院),福建南平354300

出　　处：《江苏农业学报》2020年第1期187-193,共7页Jiangsu Journal of Agricultural Sciences

基　　金：福建省科技厅项目(WYKF2017-8、2018L3018、2017Y00076);武夷学院校级课题(XP201807)

摘　　要：利用超声波辅助提取多糖的方法,研究武夷山白花葛茎多糖的最优提取工艺条件。在单因素试验基础上,以白花葛茎多糖提取率为指标,进行四因素三水平Box-Behnken设计,用响应面法分析4个因素对白花葛茎多糖提取率的影响,并进行优化。最终以6%离子液体[BMIm][PF6]3 ml、超声功率630 W、超声时间25 min、料液比1∶35(g/ml)为最佳提取条件,采用最优提取工艺条件对白花葛茎多糖进行提取,此时白花葛茎多糖的提取率为22.53 mg/g。扫描电镜观察发现,离子液体超声处理对白花葛茎粉末微观结构破坏程度较大。The optimal extraction conditions of polysaccharide from Pueraria f.niveus stem in Wuyi Mountain were studied by ultrasonic assisted extraction method.On the basis of single-factor experiment,the extraction rate of polysaccharides from the Pueraria f.niveus was determined as the index.Box-behnken was designed with four factors at three levels.Response surface method was used to analyze the effect of four factors on the extraction rate of polysaccharides from the stem of Pueraria f.niveus,and the optimization was carried out.The optimal extraction conditions were as follows:the 6%ionic liquid[BMIm][PF6]was 3.00 ml,ultrasonic power was 630 W,ultrasonic extraction time was 25 min,the solid-liquid ratio was 1∶35.Under the optimal extraction conditions,the extraction rate of polysaccharide from the stem of Pueraria f.niveus was 22.53 mg/g.Besides,the scanning electron microscope observation results showed that the microstructure of the Pueraria f.niveus was destroyed greatly by ionic liquid ultrasonic treatment.

关 键 词：白花葛 多糖 离子液体 超声波 响应面 

分 类 号：Q946.3[生物学—植物学]