晶胶固载副干酪乳杆菌及合成苯乳酸的实验研究  

Preparation of Lactobacillus paracasei cell-immobilized cryogel biocatalysts for biosynthesis of phenyllactic acid

作　　者：许娜 关今韬 张颂红 贠军贤 关怡新[2] 姚善泾[2] XU Na;GUAN Jin-tao;ZHANG Song-hong;YUN Jun-xian;GUAN Yi-xin;YAO Shan-jing(State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology,College of Chemical Engineering,Zhejiang University of Technology,Hangzhou 310032,China;College of Chemical and Biological Engineering,Zhejiang University,Hangzhou 310027,China)

机构地区：[1]浙江工业大学化学工程学院绿色化学合成技术国家重点实验室培育基地,浙江杭州310032 [2]浙江大学化学工程与生物工程学院,浙江杭州310027

出　　处：《高校化学工程学报》2020年第1期143-148,共6页Journal of Chemical Engineering of Chinese Universities

基　　金：国家自然科学基金(21576240,21036005);浙江省自然科学基金(LZ14B060001,LY16B060011)

摘　　要：针对生物法合成苯乳酸存在成本高、高产菌株缺乏、工业化困难,稳定性低等问题,利用阴离子交换晶胶对副干酪乳杆菌的吸附作用,在发酵过程中形成复合生物催化剂,用于苯乳酸发酵和转化合成。对复合催化剂微观结构、形成过程特性、发酵和转化性能进行了测定。结果表明:发酵时投入晶胶可形成固载细胞的超大孔复合生物催化剂,不仅促进菌体生长,使生物量达7.10 mg·mL^-1,是同条件下无晶胶时的2.5倍,而且通过减轻底物抑制,将苯乳酸产量由0.71 mg·mL^-1提高到0.95 mg·mL^-1;以浓度0.5、1和2 mg·mL^-1的苯丙氨酸为底物,采用复合催化剂转化生成苯乳酸的产量是相应全细胞催化剂时的3、2.5和1.5倍。Cell-immobilized cryogel biocatalysts were prepared by adsorption of Lactobacillus paracasei cells onto anion-exchange cryogels during fermentation,which were used to synthesize phenyllactic acid via fermentation and biotransformation.The microstructure,fermentation performance,and fermentation/bioconversion characteristics were investigated.The results show that super-macro porous composite biocatalysts with immobilized L.paracasei cells are formed during fermentation.The dried cell concentration in the broth was improved to 7.10 mg·mL^-1,which was 2.5 times of that under the same condition without cryogels.The phenyllactic acid concentration was also improved from 0.71 mg·mL^-1 without cryogels to 0.95 mg·mL^-1 with cryogels.Due to the reduction of substrate inhibition by cell-immobilized cryogels as the biocatalysts,the bioconversion yields from the precursor phenylalanine to phenyllactic acid were 3,2.5 and 1.5 times of whole-cell catalysts without cryogels at phenylalanine concentrations of 0.5,1 and 2 mg·mL^-1,respectively.

关 键 词：生物催化剂 苯乳酸 阴离子交换 晶胶 

分 类 号：TQ[化学工程]