河北省玉米小斑病菌优势生理小种鉴定及ITS序列分析  

Identification and ITS Sequence Analysis of Dominant Physiological Races of Bipolaris maydis in Hebei Province

作　　者：李聪聪[1] 王亚娇[1] 栗秋生[1] 纪莉景[1] 王连生[1] 孔令晓[1] LI Cong-cong;WANG Ya-jiao;LI Qiu-sheng;JI Li-jing;WANG Lian-sheng;KONG Ling-xiao(Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China,Ministry of Agriculture/IPM Center of Hebei Province/Institute of Plant Protection,Hebei Academy of Agricultural and Forestry Sciences,Baoding 071000,China)

机构地区：[1]河北省农林科学院植物保护研究所/河北省有害生物综合防治工程技术研究中心/农业部华北北部作物有害生物综合治理重点实验室

出　　处：《玉米科学》2020年第1期172-176,共5页Journal of Maize Sciences

基　　金：粮食丰产增效科技创新项目(2016YFD0300705);河北省财政基本科研业务费(2018120301)

摘　　要：2007~2016年在河北各地采集玉米小斑病标样,对分离出的356株玉米小斑病菌菌株进行生理小种鉴定。结果表明,在鉴定的年度范围内,玉米小斑病菌T小种、C小种、S生理型、O小种的分离频率在年度间存有差异,O小种的平均分离频率94.94%,是河北省玉米小斑病菌的优势小种;O小种对主栽品种郑单958和自交系C103的致病力呈下降趋势,在C103上致病力下降幅度小于郑单958。对河北省石家庄、保定、衡水、沧州邯郸、邢台采集的45株菌株进行ITS序列分析构建UPGMA进化树,分析表明,河北省内玉米小斑病菌株的遗传进化与地域有一定关系,差异不明显。Physiological races of 356 strains of Bipolaris maydis were identified, which were collected from Hebei province from 2007 to 2016. The results showed that isolation frequencies of Race T, Race C, Type S, and Race O were different from year to year. Race O is the dominant race of B.maydis in Hebei province with 94.94% average isolation frequence. Meanwhile, the pathogenicity of race O to hybrid Zhengdan958 and inbred line C103 was declining. However, the pathogenicity of Bipolaris maydis to C103 decreased less than that to Zhengdan958. ITS sequence of 45 strains collected from Shijiazhuang, Baoding, Hengshui, Cangzhou, Handan, Xingtai of Hebei province were analyzed, and UPGMA evolutionary tree was constructed. The result indicated that the genetic evolution of Bipolaris maydis was weakly geographically related.

关 键 词：玉米 玉米小斑病菌 生理小种 致病性 ITS序列分析 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]