乙醇浓度对提取灵芝三萜含量的影响及提取物抗前列腺癌细胞LNCaP的活性  

Effects of ethanol concentration on the extraction of triterpenoids in Ganoderma lingzhi and activity of the extract against prostate cancer cells LNCaP

作　　者：徐锦[2] 汪雯翰[1] 杨妍 韩伟[2] 唐传红[1] 杨焱[1] 刘艳芳[1] 张劲松[1] 贾薇[1] XU Jin;WANG Wen-Han;YANG Yan;HAN Wei;TANG Chuan-Hong;LIU Yan-Fang;ZHANG Jing-Song;JIA Wei(China Key Laboratory of Edible Fungus Resources and Utilization(South),Ministry of Agriculture,National Engineering Research Center of Edible Fungi,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;Engineering Center for Traditional Chinese Medicine Modernization,East China University of Science and Technology,Shanghai 200237,China)

机构地区：[1]农业部南方食用菌资源利用重点实验室国家食用菌工程技术研究中心上海市农业科学院食用菌研究所,上海201403 [2]华东理工大学中药现代化工程中心,上海200237

出　　处：《菌物学报》2020年第1期155-163,共9页Mycosystema

基　　金：上海市科技兴农重点攻关项目[沪农科攻字（2016）第5-4号];上海市市级农口系统青年人才成长计划[沪农青字（2016）第1-14号]~~

摘　　要：本研究考察乙醇浓度对灵芝子实体中三萜成分提取的影响及提取物抗前列腺癌细胞LNCaP的增殖活性和迁移能力,为提取灵芝三萜时乙醇浓度的选择和灵芝抑制前列腺相关疾病产品开发提供依据。研究采用高效液相(HPLC)色谱法测定了灵芝醇提物中目前具有代表性的9种三萜的含量;并用抑制前列腺癌细胞LNCaP的细胞活性和细胞迁移能力的实验研究灵芝提取物的抗前列腺癌的作用。HPLC测定结果显示,当乙醇浓度为50%–80%时,9种灵芝三萜的含量随着乙醇浓度的升高而增大;当乙醇浓度继续升高至95%时,提取的三萜含量反而有所降低。细胞实验结果显示,4个浓度乙醇的灵芝提取物都具有良好的抑制LNCaP肿瘤细胞增殖作用和阻滞LNCaP细胞迁移能力。因此在使用乙醇提取灵芝三萜时,乙醇浓度升高有利于三萜的提取;在生产灵芝抗前列腺相关产品时,提取灵芝子实体中抗前列腺增生的活性成分时选用80%的乙醇较为适宜。Effects of ethanol concentration on the extraction of triterpenoids in Ganoderma lingzhi and the activity of the extract against prostate cancer cell LNCaP are evaluated.Nine representative triterpenoids in Ganoderma lingzhi were determined with the help of HPLC.The activities of extracts obtained under different ethanol concentration inhibiting LNCaP from proliferation and migration were determined.HPLC results showed that under ethanol concentration of 50%–80%,the content of nine triterpenoids increased with the increase of ethanol concentration.The content of triterpenoids decreased when ethanol concentration attained to 95%.Cell experiments showed that all ethanol extracts obtained under different ethanol concentration had a good effect in inhibiting the proliferation and retarding the migration of LNCaP,and concentration of 80%ethanol is more appropriate for the production of Ganoderma lingzhi products with anti-prostate cancer activity.

关 键 词：三萜 乙醇浓度 LNCAP细胞 化学法 HPLC测定 

分 类 号：R285.5[医药卫生—中药学]