基质金属蛋白酶-11在结肠腺癌中的表达及与微淋巴管密度的相关性  

Expression of MMP-11 incolorectal adenocarcinomaand its relationship with microlymphatic vessel density

作　　者：刘爱东 马征[2] 庞久玲[3] 刘时飞 闫丰 Liu Aidong;Ma Zheng;Pang Jiuling;Liu Shifei;Yan Feng(Department of Pathology,the Affiliated Hospital of North China University of Science and Technology,Tangshan 063000,China;Medical College,Tangshan Vocational and Technical College,Tangshan 063000,China;Department of Pathology,Tangshan Workers's Hospital,Tangshan 063000,China)

机构地区：[1]华北理工大学附属医院病理科,河北唐山063000 [2]唐山职业技术学院医学校区,河北唐山063000 [3]唐山市工人医院病理科,河北唐山063000

出　　处：《临床荟萃》2020年第1期63-66,共4页Clinical Focus

基　　金：唐山市科技局课题——基于结肠腺癌中EIF2a表达的相关实验研究(19150207E);河北省卫健委医学科学研究课题计划——结肠腺癌中EIF2a的表达及与K-ras基因突变关系的研究(20200643)

摘　　要：目的检测基质金属蛋白酶-11(MMP-11)在结肠腺癌中的表达,探讨其与血管内皮生长因子-C(VEGF-C)和D2-40标记的微淋巴管密度(MLVD)的相关性。方法58例结肠腺癌根治术患者,以肿瘤组织作为观察组,以正常结肠黏膜组织作为对照组。采用免疫组化法检测MMP-11和D2-40的表达。采用Western Blot法检测VEGF-C的表达。结果两组中MMP-11的阳性率、MLVD值差异有统计学意义。观察组中MMP-11、MLVD与脉管癌栓、淋巴结转移及浸润深度相关。MMP-11的表达与预后有关。MMP-11与MLVD、MMP-11与VEGF-C均具有正相关性。结论结肠腺癌组织中MMP-11的表达升高,间质中MLVD增高,对病变的发生和进展有重要作用。MMP-11和VEGF-C、MMP-11和MLVD有明显的正向协同作用,MMP-11可能通过促进淋巴管生成对肿瘤生物学行为起作用。Objective To detect the expression of matrix metalloproteinase 11(MMP-11)in colorectal adenocarcinoma,explore its correlation with vascular endothelial growth factor C(VEGF-C)and microlymphatic vessel density(MLVD)marked by D2-40.Methods Fifty eight patients with radical resection of colorectal adenocarcinoma were collected.Neoplasm tissues were collected as observation group.Normal mucosa were collected as control group.Expressions of MMP-11 and D2-40 in two groups were detected by immunohistochemistry method.And the expression of VEGF-C was detected by Western blot method.Results There were statistically significant differences in positive rate of MMP-11 and MLVD value between two groups.In the observation group,MMP-11 and MLVD were correlated with vascular cancer thrombus,lymph node metastasis and invasive degree.The expression of MMP-11 related to prognosis.Positive correlation were found between MMP-11 and MLVD,MMP-11 and VEGF-C.Conclusion The increase of MMP-11 expression and MLVD plays an important role in the occurrence and progression of colorectal adenocarcinoma.MMP-11 and VEGF-C,MMP-11 and MLVD had obvious positive synergistic effects.MMP-11 may play a role in the tumorbiological behaviorsby promoting lymphangiogenesis.

关 键 词：结肠肿瘤 腺癌 淋巴转移 基质金属蛋白酶11 血管内皮生长因子C 

分 类 号：R735.35[医药卫生—肿瘤]