白及种子苗与组培苗的生长及生理特性  

Growth and Physiological Characteristics of Seed Seedling and Tissue Culture Seedling in Bletilla striata

作　　者：梁莹[1] 林杨[1] 林伟[1] 蔡锦源[2] 郭晓云[1] 韦坤华[1] LIANG Ying;LIN Yang;LIN Wei;CAI Jinyuan;GUO Xiaoyun;WEI Kunhua(Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement,Guangxi Botanical Garden of Medicinal Plants,Nanning,Guangxi 530023;Lushan College,Guangxi University of Science and Technology,Liuzhou,Guangxi 545616,China)

机构地区：[1]广西药用植物园,广西药用资源保护与遗传改良重点实验室,广西南宁530023 [2]广西科技大学鹿山学院,广西柳州545616

出　　处：《贵州农业科学》2020年第2期88-91,共4页Guizhou Agricultural Sciences

基　　金：国家中医药行业科研专项“20种道地药材的优良品种选育方法及种植质量控制技术研究”(201507002);国家中药材产业技术体系“南宁综合试验站”(CARS-21);中央引导地方科技发展专项“药用植物资源保存能力提升工程”(桂科ZY1949023);广西科技计划项目“珍稀濒危药用资源保护与可持续利用工作站”(桂科AD17129044);“广西八桂学者”专项经费项目

摘　　要：为构建白及种苗的繁育技术体系及其产业化生产示范提供理论依据,采用大棚人工栽培方法研究白及种子苗与组培苗的农艺性状和生理特性差异。结果表明:白及种子苗与组培苗的各项农艺性状指标值均随生长时间延长呈上升趋势,同一苗龄白及组培苗长势高于种子苗,组培苗6月龄、9月龄和12月龄的叶长、叶宽、鳞茎长和鳞茎宽分别较同期种子苗增加1.10~4.91 cm、0.41~1.15 cm、0.10~5.75 mm和0.94~1.66 mm;其鳞茎抗氧化酶活性优于种子苗,组培苗6月龄、9月龄和12月龄的过氧化物酶活性分别较同期种子苗增加9.72 U/(g FW·min)、0.35 U/(g FW·min)和-3.61 U/(g FW·min),超氧化物歧化酶活性分别增加202.77 U/(g FW·min)、145.84 U/(g FW·min)和86.59 U/(g FW·min);但其茎叶各项生理生化指标与种子苗的差异不明显。从生产成本考虑,白及种子苗可能成为目前白及种苗生产的主要来源。The difference in agronomic traits and physiological characteristics of seed seedlings and tissue culture seedlings in Bletilla striata is compared by an artificial cultivation method in greenhouse to provide the theoretical basis for propagation technical system and industrialization production of B.striata seedlings.Result:The various agronomic trait indexes of seed seedlings and tissue culture seedlings show a rising trend with extension of growth time.The growth vigor of tissue culture seedlings is higher than seed seedlings under the same seedling age and the leaf length,leaf width,bulb length and bulb width of tissue culture seedlings with six-month age,ninemonth age and one-year age are 1.10-4.91 cm,0.41-1.15 cm,0.10-5.75 mm and 0.94-1.66 mm higher than seed seedlings with six-month age,nine-month age and one-year age respectively.The bulb peroxidase activity of tissue culture seedlings with six-month age and nine-month age is 9.72 U/(g FW·min)and 0.35 U/(g FW·min)higher than seed seedlings with six-month age and nine-month age separately but the bulb peroxidase activity of tissue culture seedlings with one-year age is 3.61 U/(g FW·min)lower than seed seedlings with oneyear age.The bulb superoxide dismutase activity of tissue culture seedlings with six-month age,nine-month age and one-year age are 202.77 U/(g FW·min),145.84 U/(g FW·min)and 86.59 U/(g FW·min)higher than seed seedlings with six-month age,nine-month age and one-year age respectively.There are no obvious differences in various biochemical indexes of stem and leaf between seed seedlings and tissue culture seedlings.The seed seedlings may be the main source in production of B.striata seedlings considering the production cost at present.

关 键 词：白及 种子苗 组培苗 生产性状 比较 

分 类 号：S567.23[农业科学—中草药栽培]