侵染贵州火龙果的仙人指X病毒的基因组序列变异  

Genome Sequence Variation of Schlumbergera virus X ( SchVX) Infecting Pitaya in Guizhou

作　　者：郑乾明 王小柯 马玉华[1] ZHENG Qianming;WANG Xiaoke;MA Yuhua(Institute of Fruit Sciences,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006,China)

机构地区：[1]贵州省农业科学院果树科学研究所

出　　处：《贵州农业科学》2020年第2期95-98,共4页Guizhou Agricultural Sciences

基　　金：贵州省农业科学院青年基金项目“利用高通量测序挖掘火龙果病毒”[黔农科院青年基金(2017)11];贵州省第六批人才基地项目“贵州省精品水果产业人才培养基地”(RCJD2018-16)

摘　　要：为仙人指X病毒(Schlumbergera virus X,SchVX)的分子检测与防控提供理论基础,利用基于Illumina平台的高通量测序分析6份疑似病毒感染贵州火龙果样品,研究侵染火龙果的仙人指X病毒发生情况和遗传变异。结果表明:在5份样品中获得大量SchVX相关序列,占样品序列数的3.7%~17.5%;SchVX贵州分离物与参考基因组(Genbank登录号:KP090203)的序列一致性为82.8%~100.0%;SchVX贵州分离物之间编码外壳蛋白的核苷酸序列和推导的氨基酸序列一致性分别为92.5%~97.3%和96.0%~99.1%,分别与来自于仙人掌科其他寄主的NI、K11和Palma-PE分离物序列一致性类似,略高于nopal verdure 1分离物;SchVX贵州分离物与NI、K11和Palma-PE分离物亲缘关系较近,与nopal verdure 1分离物较远。样品采集地的火龙果普遍受SchVX侵染,其基因组序列存在一定程度的分子变异。To study the occurrence and genetic variation of Schlumbergera virus X(SchVX)infecting pitayas(Hylocereus.spp)from Guizhou province,six suspected samples infected with virus were subjected to highthroughput sequencing using the Illumina platform to provide the theoretical basis for molecular detection and prevention of SchVX.The results showed that a large number of SchVX-related sequences were obtained from five samples,which accounted for 3.7%-17.5%of the total sequences of each sample.The sequence identities of SchVX Guizhou isolates with the reference genome(Genbank accession:KP090203)were 82.8%-100.0%.The nucleotide sequence and deduced amino acid sequence identities of the coat proteins from SchVX Guizhou isolates were 92.5%-97.3%and 96.0%-99.1%,respectively,which were similar to NI,K11 and Palma-PE isolates from other hosts of the Cactus family,and slightly higher than nopal verdure 1.Phylogenetic analysis suggested that SchVX Guizhou isolates were closely related to NI,K11 and Palma-PE isolate,and far from nopal verdure 1.This study suggested that pitayas from sample collection sites were generally infected by SchVX,which had a certain degree of molecular variation in its genome sequences.

关 键 词：高通量测序 系统进化 外壳蛋白 分子变异 火龙果 

分 类 号：S432.41[农业科学—植物病理学]