GFAP expression in the optic nerve and increased Н2S generation in the integration centers of the rainbow trout（Oncorhynchus mykiss） brain after unilateral eye injury  

作　　者：Evgeniya V.Pushchina Anatoly A.Varaksin Dmitry K.Obukhov Igor M.Prudnikov 

机构地区：[1]A.V.Zhirmunsky National Scientific Center of Marine Biology,Far Eastern Branch,Russian Academy of Sciences,Vladivostok,Russia [2]A.A.Bogomoletz Institute of Physiology,National Academy of Sciences of Ukraine,Kiev,Ukraine [3]St.Petersburg State University,St.Petersburg,Russia

出　　处：《Neural Regeneration Research》2020年第10期1867-1886,共20页中国神经再生研究（英文版）

基　　金：supported by a grant from the President of the Russian Federation(No.MD-4318.2015.4; to EVP);a grant from the Program for Basic Research of the Far East Branch of the Russian Academy of Sciences 2015–2017(No.15-I-6-116,section III to EVP,AAV and DKO)

摘　　要：Hydrogen sulfide(H2S)is considered as a protective factor against cardiovascular disorders.However,there are few reports on the effects of H2S in the central nervous system during stress or injury.Previous studies on goldfish have shown that astrocytic response occurs in the damaged and contralateral optic nerves.Glial fibrillary acidic protein(GFAP)concentration in the optic nerves of rainbow trout has not been measured previously.This study further characterized the astrocytic response in the optic nerve and the brain of a rainbow trout(Oncorhynchus mykiss)after unilateral eye injury and estimated the amount of H2S-producing enzyme cystathionineβ-synthase(CBS)in the brain of the rainbow trout.Within 1 week after unilateral eye injury,a protein band corresponding to a molecular weight of 50 kDa was identified in the ipsi-and contralateral optic nerves of the rainbow trout.The concentration of GFAP in the injured optic nerve increased compared to the protein concentration on the contralateral side.The results of a quantitative analysis of GFAP+cell distribution in the contralateral optic nerve showed the largest number of GFAP+cells and fibers in the optic nerve head.In the damaged optic nerve,patterns of GFAP+cell migration and large GFAP+bipolar activated astrocytes were detected at 1 week after unilateral eye injury.The study of H2S-producing system after unilateral eye injury in the rainbow trout was conducted using enzyme-linked immunosorbent assay,western blot analysis,and immunohistochemistry of polyclonal antibodies again st CBS in the integrative centers of the brain:telencephalon,optic tectum,and cerebellum.Enzyme-linked immunosorbent assay results showed a 1.7-fold increase in CBS expression in the rainbow trout brain at 1 week after unilateral eye injury compared with that in intact animals.In the ventricular and subventricular regions of the rainbow trout telencephalon,CBS+radial glia and neuroepithelial cells were identified.After unilateral eye injury,the number of CBS+neuroepithelial cells in theHydrogen sulfide（H2 S） is considered as a protective factor against ca rdiovascular disorders.However,there are few reports on the effects of H2 S in the central nervous system during stress or injury.Previous studies on goldfish have shown that astrocytic response occurs in the damaged and contralateral optic nerves.Glial fibrillary acidic protein（GFAP） concentration in the optic nerves of rainbow trout has not been measured previously.This study further characterized the astrocytic response in the optic nerve and the brain of a rainbow trout（Oncorhynchus mykiss） after unilateral eye injury and estimated the amount of H2 S-producing enzyme cystathionine β-synthase（CBS） in the brain of the rainbow trout.Within 1 week after unilate ral eye injury,a protein band corresponding to a molecular weight of 50 kDa was identified in the ipsi-and contralateral optic nerves of the rainbow trout.The concentration of GFAP in the injured optic nerve increased compared to the protein concentration on the contralateral side.The results of a quantitative analysis of GFAP+cell distribution in the contralateral optic nerve showed the largest number of GFAP+cells and fibe rs in the optic nerve head.In the damaged optic nerve,patterns of GFAP+cell migration and large GFAP+bipolar activated astrocytes were detected at 1 week after unilateral eye injury.The study of H2 S-producing system after unilateral eye injury in the rainbow trout was conducted using enzyme-linked immunosorbent assay,western blot analysis,and immunohistochemistry of polyclonal antibodies again st CBS in the integrative centers of the brain:telencephalon,optic tectum,and ce rebellum.Enzyme-lin ked immunosorbent assay results showed a 1.7-fold increase in CBS expression in the rainbow trout brain at 1 week after unilateral eye injury com pared with that in intact animals.In the ventricular and subventricular regions of the rainbow trout telencephalon,CBS+radial glia and neu roepithelial cells were identified.After unilateral eye injury,the number of

关 键 词：astrocyte-like cells glial fibrillary acidic protein hydrogen sulfide neuroepithelial cells NEUROPROTECTION radial glial cells rainbow trout(Oncorhynchus mykiss) reactive oxygen species unilateral eye injury 

分 类 号：R446[医药卫生—诊断学] R364[医药卫生—临床医学]