Gold immunochromatographic assay for simultaneous detection of sibutramine and sildenafil in slimming tea and coffee  

胶体金免疫层析分析方法用于同时检出减肥茶和咖啡中西布曲明和西地那非（英文）

作　　者：Steven Suryoprabowo Liqiang Liu Hua Kuang Gang Cui Chuanlai Xu Steven Suryoprabowo;刘丽强;匡华;崔钢;胥传来(State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China;International Joint Research Laboratory for Biointerface and Biodetection,and School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;School of Life Sciences,Yancheng Teachers University,Yancheng 224002,China)

机构地区：[1]State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China [2]International Joint Research Laboratory for Biointerface and Biodetection,and School of Food Science and Technology,Jiangnan University,Wuxi 214122,China [3]School of Life Sciences,Yancheng Teachers University,Yancheng 224002,China

出　　处：《Science China Materials》2020年第4期654-659,共6页中国科学（材料科学（英文版）

基　　金：financially supported by the National Key R&D Program of China (2016YFD0401101 and KYCX18_1756)

摘　　要：Weight gain and obesity are worldwide problems prompting a search for many health supplements that provide benefits such as revitalization,skin-beautification,visual improvement,and slimming effects,mainly based on herbal formulations as an alternative treatment.Obesity may be defined as decreased physical activity,energy intake greater than energy expenditure,poor eating habits,neurogenic abnormalities,and genetic factors that have negative impact on the quality of life[1-3].Sibutramine(SB)is one of the most widely used herbal formulations or dietary supplements used for weight loss.It has also been used to treat polycystic ovary syndrome,and usually found in anorexic drugs used in herbal slimming[4-6].本文建立了一种方便、快速、高特异性的胶体金免疫层析检测方法,用于同时检测减肥茶和咖啡中的西布曲明(SB)和西地那非(SID).我们首先制备了胶体金纳米粒子(CG),然后使之与单抗结合形成CG-mAb,将CG-mAb与抗原分别喷涂到基板上形成结合垫和检测条带,其中所需的抗原和抗体均由本实验室制备.经过条件优化,该方法在PBS缓冲溶液(0.01 mol L^-1,pH7.4),以及三种减肥茶和三种咖啡样本中,对SB和SID的消线值均为500 ng mL^-1;检测时间仅需5 min.该方法对减肥茶和咖啡样本中SB和SID的快速检出具有实际应用价值.

关 键 词：高特异性 减肥茶 西地那非 西布曲明 胶体金免疫层析 实验室制备 金纳米粒子 咖啡 

分 类 号：O658.1[理学—分析化学] TS272.7[理学—化学] TS273[农业科学—茶叶生产加工]