莽吉柿提取物对宫颈癌Hela细胞凋亡的影响  

Effect of Extract of Garcinia Mangostana on Apoptosis of Hela Cells

作　　者：李凯强 郝珂[2] 陈以栗[3] 陈秉宇 蒋珊珊[2] 张威[2] 柴可群[1,4] LI Kaiqiang;HAO Ke;CHEN Yili;CHEN Bingyu;JIANG Shanshan;ZHANG Wei;CAI Kequn(Second Clinical Medical College,Zhejiang Chinese Medical University,Hangzhou,Zhejiang province,310053,China;Department of Blood Transfusion,Zhejiang Provincial People's Hospital,Hangzhou,Zhejiang province,310014,China;School of Pharmacy,Zhejiang University of Technology,Hangzhou,Zhejiang province,310014,China;Department of Oncology,Tongde Hospital of Zhejiang Province,Hangzhou,Zhejiang province,310012,China)

机构地区：[1]浙江中医药大学第二临床医学院,杭州310053 [2]浙江省人民医院(杭州医学院附属人民医院)输血科,杭州310014 [3]浙江工业大学药学院,杭州310014 [4]浙江省立同德医院肿瘤科,杭州310012

出　　处：《浙江中西医结合杂志》2020年第2期109-112,I0003,共5页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省自然科学基金(No.LQ17H160017)。

摘　　要：目的研究莽吉柿提取物对宫颈癌Hela细胞凋亡的影响。方法使用不同浓度0.75、1.25、2.5、5μg/mL的莽吉柿提取物处理体外培养Hela细胞,CCK-8检测试剂盒检测其对宫颈癌细胞增殖的影响;流式细胞术检测细胞凋亡和细胞活性氧(ROS)表达水平;Western Blot检测磷酸化的细胞外调节蛋白激酶(p-ERK1/2)、B淋巴细胞瘤-2蛋白(Bcl-2)和Bcl-2相关X蛋白(BAX)表达水平。结果莽吉柿提取物对宫颈癌细胞的增殖具有显著的抑制作用(P<0.05),IC50为1.63μg/mL。流式细胞术检测发现,莽吉柿提取物处理组(2.5μg/mL和5μg/mL)的Hela细胞的凋亡率显著高于对照组[(4.19±0.24)%、(14.03±0.21)%比(3.30±0.10)%,P均<0.05]。莽吉柿提取物处理组的ROS含量显著高于对照组,为对照组的3.05倍,P<0.05。荧光显微镜结果显示莽吉柿提取物处理组的ROS荧光强度显著升高。Western Blot检测发现,莽吉柿提取物处理组(2.5μg/mL和5μg/mL)宫颈癌Hela细胞p-ERK1/2蛋白表达量显著低于对照组,分别为对照组的54%和25%;Bcl-2蛋白表达量显著低于对照组,分别为对照组的76%和67%;BAX蛋白表达量显著高于对照组,分别为对照组的1.11倍和1.45倍(P均<0.05)。结论莽吉柿提取物对宫颈癌细胞的影响可能是通过诱导细胞氧化损伤,调控肿瘤细胞MAPK/ERK1/2信号通路和凋亡相关蛋白,进而诱导细胞发生凋亡,抑制肿瘤细胞的增殖。Objective To explore the effect of extract of Garcinia mangostana on apoptosis of cervical cancer HeLa cells. Methods HeLa cells cultured in vitro were treated with different concentrations of extract of Garcinia mangostana, 0.75, 1.25, 2.5, and 5μg/mL. CCK-8 detection kit was used to check its effect on cell proliferation of cervical cancer. Flow cytometry was used to test apoptosis and ROS expression. Western Blot was performed to detect the expression levels of phosphorylated extracellular signal-regulated kinase(p-ERK1/2), B cell CLL/lymphoma2(Bcl-2) and Bcl-2 related X protein(BAX). Results Extract of Garcinia mangostana had a significant inhibitory effect on the proliferation of HeLa cells(P<0.05), with IC50 of 1.63μg/mL. The apoptosis rate of HeLa cells in extract of Garcinia mangostana-treated group(2.5 and 5μg/mL) was significantly higher than that in the control group[(4.19±0.24)% and(14.03±0.21)%, vs(3.30±0.10)%, P<0.05]. ROS in extract of Garcinia mangostana-treated group was 3.05 folds higher than that in control group(P<0.05). ROS fluorescence intensity in extract of Garcinia mangostana-treated group increased significantly under fluorescence microscope. Western blot analysis showed that the expression of p-ERK1/2 in extract of Garcinia mangostana-treated group(2.5 and 5μg/mL) was significantly lower, only 54% and 25% of the control group, respectively. And so was the expression of Bcl-2, only 76% and67% of the control group, respectively. The expression of BAX was 1.11 and 1.45 folds higher than that of the control group, respectively(P<0.05). Conclusion The effect of extract of Garcinia mangostana on cervical cancer cells may be through inducing oxidative damage of cells, regulating MAPK/ERK1/2 signal pathway and apoptosisrelated proteins, thus inducing apoptosis of cells and inhibiting proliferation of tumor cells.

关 键 词：宫颈癌 莽吉柿提取物 凋亡 ROS 

分 类 号：R285[医药卫生—中药学]