肿瘤坏死因子-α基因多态性与肝细胞癌遗传易感性的关联研究  

Association between gene polymorphism of TNF-α and genetic susceptibility to hepatocellular carcinoma

作　　者：申鹏[1] 翁美玲[2] 汪正飞[1] SHEN Peng;WENG Meiling;WANG Zhengfei(Department of Hepatobiliary Surgery,Quzhou People's Hospital,Quzhou 324000,China)

机构地区：[1]衢州市人民医院肝胆外科,浙江衢州324000 [2]衢州市人民医院肿瘤内科,浙江衢州324000

出　　处：《全科医学临床与教育》2020年第2期121-124,共4页Clinical Education of General Practice

摘　　要：目的探究肿瘤坏死因子-α(TNF-α)基因单核苷酸多态性(SNP)位点的多态性与肝细胞癌(HCC)遗传易感性的相关性。方法选取HCC患者112例作为实验组,并选取同期住院无血缘关系的非肿瘤患者100例作为对照组。采用Taqman探针荧光定量PCR法对TNF-α基因多态性位点-238G/A和-308A/G进行基因分型,观察两多态性位点的基因型及等位基因频率在两组间的分布特征,并采用logistic回归分析多态位点与HCC的相关性。结果实验组患者血清TNF-α水平明显高于对照组,差异有统计学意义(t=13.36,P<0.05);在TNF-α基因-238G/A位点中,两组间基因型及等位基因频率分布比较,差异均无统计学意义(χ2分别=1.61、0.02,P均>0.05);在TNF-α基因-308A/G位点中,实验组AA基因型频率明显低于对照组,G等位基因频率明显高于对照组,差异均有统计学意义(χ2分别=6.46、7.23,P均<0.05);-308A/G位点中,AG+GG基因型的患者血清TNF-α水平明显高于AA基因型,差异有统计学意义(t=6.10,P<0.05)。结论 TNF-α基因多态性与HCC之间可能存在一定的相关性,其中-308A/G位点中的G等位基因可能是HCC遗传易感性的风险等位基因,监测TNF-α基因中-308A/G位点的多态性对及时准确地预测HCC的发生、改善HCC患者预后具有重要的临床意义。Objective To explore the correlation between TNF-α gene polymorphism and genetic susceptibility to hepatocellular carcinoma(HCC). Methods Totally 112 patients with HCC were selected as the experimental group,and100 patients without blood relationship and tumor were selected as the control group.The Taqman probe quantitative PCR method was used togenotype TNF-α gene polymorphism loci-238 G/A and-308 A/G,and the distribution characteristics of the genotypes and allele frequencies of the two polymorphism loci between the two groups were observed.And the correlation between the polymorphism loci and hepatocellular carcinoma was analyzed by logistic regression. Results The serum TNF-α level in the experimental group was significantly higher than that in the control group(t=13.36,P<0.05).In the TNF-α gene-238 G/A locus,there was no significant difference in genotype and allele frequency distribution between the two groups(χ2=1.61,0.02,P>0.05).In the TNF-α gene-308 A/G locus,the AA genotype frequency in the experimental group was significantly lower than that in the control group,while the G allele frequency was significantly higher than the control group(χ2=6.46,7.23,P<0.05).In the-308 A/G locus,the serum TNF-α level in the patients with AG+ GG genotype was significantly higher than that in the patients with AA genotype,the difference was statistically significant(t=6.10,P<0.05). Conclusion TNF-α gene polymorphism may be has a certain correlation with HCC,and G allele of-308 A/G locus may be a risk allele for genetic susceptibility to HCC.Monitoring the 308 A/G loci polymorphism of TNF-α is important to the accurate prediction of the occurrence of HCC and the prognosis of patients with HCC.

关 键 词：肿瘤坏死因子-Α 肝细胞癌 多态性 基因 遗传易感性 相关性 

分 类 号：R735.7[医药卫生—肿瘤]