中药萎胃康通过影响Hedgehog通路治疗慢性萎缩性胃炎的机制研究  

Mechanism of Weiweikang in Treating Chronic Atrophic Gastritis by Affecting Hedgehog Pathway

作　　者：陈露 于佳宁 吕俊慧 宋汉娜 李俊材 林海燕[1] CHEN Lu;YU Jia-ning;LV Jun-hui;SONG Han-na;LI Jun-cai;LIN Hai-yan(College of Combining Traditional Chinese and Western Medicine,Binzhou Medical University,Shandong Yantai 264003,China;Two Gland Surgery,Yantai Affiliated Hospital of Binzhou Medical University,Shandong Yantai 264100,China)

机构地区：[1]滨州医学院中西医结合学院,烟台264003 [2]滨州医学院烟台附属医院两腺外科,山东烟台264100

出　　处：《中国中医基础医学杂志》2019年第12期1668-1672,共5页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：山东省自然科学基金资助项目(ZR2014HL094)-基于Hedgehog信号通路变化的中药萎胃康颗粒治疗慢性萎缩性胃炎癌前病变的机制研究;山东省中医药科技发展计划项目(2017-235)-益气养阴活血法治疗慢性萎缩性胃炎的Wnt/β-catenin机制研究。

摘　　要：目的:探讨萎胃康对慢性萎缩性胃炎的治疗作用及机制。方法:采用水杨酸钠溶液灌胃和饥饱失常为主的多重刺激法进行造模成功后,中药萎胃康以高(3 g/kg/d)、中(1. 5 g/kg/d)、低(0. 75 g/kg/d)剂量治疗;西药组以维酶素(0. 3 g/kg/d)来治疗,模型组和正常组使用生理盐水进行灌胃。H-E染色观察胃组织的病理学改变;免疫组化法检测在治疗第10天,20天,30天时各组大鼠胃黏膜的圆滑蛋白基因Smoothened(Smo)、音猬因子SonicHedgehog(Shh)和丝氨酸/苏氨酸蛋白激酶Fused(Fu)抑制剂(SuFu)的蛋白表达情况。经过治疗后大鼠的病理变化有所好转,与正常组比较模型组大鼠胃黏膜的Smo、Shh和SuFu蛋白表达量增多,与模型组比较各治疗组大鼠胃黏膜的各蛋白表达量下降,与西药组比较中药高剂量组的各蛋白表达量下降明显。结论:萎胃康治疗慢性萎缩性胃炎疗效显著,且高剂量组疗效优于西药,其治疗慢性萎缩性胃炎的机制可能是与减少Smo、Shh和SuFu的表达量有关。Objective To explore the therapeutic effects and mechanisms of Weiweikang in treating chronic atrophic gastritis(CAG).Methods The rat model of CAG was established by the sodium salicylate and the irregular diet.After the model was made successfully,Weiweikang high(3 g/kg/d)、medium(1.5 g/kg/d)、low dose group(0.75 g/kg/d)and Victoria Su-enzyme group(0.3 g/kg/d)were threated CAG;The model group and the normal group were received intragastric administration with normal saline.The morphological changes of gastric mucosa were observed by H-E method.Immunohistochemistry(IHC)was used to observe the changes of Smoothened(Smo)、Sonic Hedgehog(Shh)and Inhibitors of serine/threonine protein kinase(Su Fu)in gastric mucosa of rats on day 10,20,and 30.Results The pathological changes of gastric tissue were improved after treatment;Compared with the normal group,the expression levels of Smo、Shh and Su Fu in the gastric mucosa of rats were increased in the model group;Compared with the model group,the protein expression of gastric mucosa decreased in each treatment group;Compared with Victoria Su-enzyme group,the protein expression of each group in the Weiweikang high-dose group was decreased.Conclusion Weiweikang and Victoria Su-enzyme can threat CAG effectively,the effect of the high-dose group was greater than Victoria Su-enzyme,the mechanism may be related to the reduction of abnormal expression of Smo、Shh and Su Fu in the gastric mucosa.

关 键 词：慢性萎缩性胃炎 萎胃康 HEDGEHOG通路 

分 类 号：R573.32[医药卫生—消化系统]