微透析/液相色谱-质谱联用筛选黄芩中α-葡萄糖苷酶抑制剂  

Screening ofα-Glucosidase Inhibitors in Scutellaria Baicalensis by Liquid Chromatography-Mass Spectrometry with Microdialysis

作　　者：庞博 王倩倩 刘舒[1] 刘志强[1] 宋凤瑞[1,2] PANG Bo;WANG Qian-qian;LIU Shu;LIU Zhi-qiang;SONG Feng-rui(National Center of Mass Spectrometry in Changchun,Key Laboratory of Traditional Chinese Medicine Chemistry and Mass Spectrometry Jilin Province,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China;School of Applied Chemistry and Engineering,University of Science and Technology for China,Hefei 230026,China;State Key Laboratory of Medicinal Chemical Biology,Nankai University,Tianjin 300457,China)

机构地区：[1]中国科学院长春应用化学研究所,长春质谱中心&吉林省中药化学与质谱重点实验室,吉林长春130022 [2]中国科学技术大学应用化学与工程学院,安徽合肥230026 [3]南开大学药物化学生物学国家重点实验室,天津300457

出　　处：《分析测试学报》2020年第1期157-161,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(21673219,81373952)。

摘　　要：应用微透析/液相色谱-质谱联用法筛选黄芩中α-葡萄糖苷酶抑制剂,并对筛选出的具有酶抑制活性的化合物进行结构鉴定。实验采用迪马C18色谱柱(150 mm×4.6 mm,5μm),以0.5%醋酸水-乙腈溶液为流动相,梯度洗脱,流速0.5 mL/min,柱温30℃,以正离子电喷雾质谱模式采集数据,从黄芩中筛选出10种潜在的α-葡萄糖苷酶抑制剂,10种化合物与α-葡萄糖苷酶的结合率为36.18%~52.71%。该方法可快速、有效地筛选黄芩中的α-葡萄糖苷酶的抑制剂,同时可为从其他中药中筛选酶抑制剂提供方法学借鉴。A liquid chromatography-mass spectrometry with microdialysis was used to screen potentialα-glucosidase inhibitors in the extracts of Scutellaria baicalensis and identify the structures of those bioactive components screened.The chromatographic separation was carried out on a Dikma C18 column(150 mm×4.6 mm,5μm)by gradient elution with 0.5%acetic acid solution-acetonitrile as mobile phases at a flow rate of 0.5 mL/min and a column temperature of 30℃.Mass spectrometry analysis and data acquisition were conducted in the positive ion mode.In this work,ten potentialα-glucosidase inhibitors in Scutellaria baicalensis were found and identified simultaneously.The binding degrees of 10 compounds withα-glucosidase ranged from 36.18%to 52.71%.With the advantages of rapidness,high throughput and effectiveness,this method could screenα-glucosidase inhibitors in Scutellaria baicalensis and provide valuable information for screeningα-glucosidase inhibitors from other traditional Chinese medicines.

关 键 词：黄芩 Α-葡萄糖苷酶 微透析 HPLC-MS^N 抑制剂 

分 类 号：O657.6[理学—分析化学] O657.72[理学—化学]