高效毛细管电泳法同时分离测定化妆品中7种防腐剂  

Simultaneous Separation and Determination of Seven Preservatives in Cosmetics by High Performance Capillary Electrophoresis

作　　者：张帅 高立娣[1] 梁辉 秦世丽 蒋春红 段圣村 徐蕾[1] 林肖同 王媛媛[1] ZHANG Shuai;GAO Li-di;LIANG Hui;QIN Shi-li;JIANG Chun-hong;DUAN Sheng-cun;XU Lei;LIN Xiao-tong;WANG Yuan-yuan(College of Chmistry and Engineering,Qiqihar University,Qiqihar 161006,China;Biotechnology Bioengineering Co.,Ltd,Shanghai 201611,China;Shanghai Bailao Daily Chemistry Co.,Ltd,Shanghai 200040,China)

机构地区：[1]齐齐哈尔大学化学与化学工程学院,黑龙江齐齐哈尔161006 [2]生工生物工程(上海)股份有限公司,上海201611 [3]上海百雀羚日用化学有限公司,上海200040

出　　处：《分析测试学报》2020年第2期258-262,共5页Journal of Instrumental Analysis

基　　金：黑龙江省教育厅海外学人科研项目(1254HQ012);黑龙江省教育厅基本业务专项理工面上项目(135209215);齐齐哈尔市科学技术计划项目(SFGG-201920);黑龙江省自然科学基金项目(B2016009)。

摘　　要：建立了毛细管电泳(CE)法同时分离测定化妆品中7种防腐剂(对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯、对羟基苯甲酸丁酯、氯苯甘醚、辛酰羟戊酸、脱氢乙酸钠)的方法。考察了添加剂种类及浓度,磷酸盐缓冲液pH值及分离电压对分离的影响。结果表明:在pH 8.0的20 mmol/L磷酸盐缓冲液中添加10 mmol/L磺丁基-β-环糊精,以及20 kV分离电压下,7种防腐剂可在5 min内实现基线分离。在5~200 mg/L质量浓度范围内,7种防腐剂呈良好线性,且相关系数(r)为0.9907~0.9989,检出限为1.23~4.30 mg/L,迁移时间的相对标准偏差(RSD)不大于1.3%,样品回收率为95.4%~103%。该方法准确、简单、高效、实用,可用于实际样品中防腐剂的测定。A capillary electrophoresis(CE)method was established for the simultaneous separation and determination of seven preservatives in cosmetics,including methyl p-hydroxybenzoate,ethyl p-hydroxybenzoate,propyl p-hydroxybenzoate,butyl p-hydroxybenzoate,chlorophenylglycol,octanoyl valerate and sodium dehydroacetate.Effects of different electrophoresis conditions,including type and concentration of additive,pH value of the running buffer and separation voltage on separation were investigated.The optimal conditions were follows:additive:10 mmol/L sulfobutyl-β-cyclodextrin,buffer:pH 8.020 mmol/L phosphate buffer,separate voltage:20 kV,detection wavelength:280 nm.Results showed that,under the optimal conditions,seven preservatives could reach the baseline separation within 5 min.The calibration curves were linear in the concentration range of 5-200 mg/L,with correlation coefficients(r)of 0.9907-0.9989.The detection limits were in the range of 1.23-4.30 mg/L,and the relative standard deviations(RSD)for migration time were not more than 1.3%.Average recoveries for the spiked samples were in the range of 95.4%-103%.The method is accurate,simple,efficient and practical,and could be applied in the determination of seven preservatives in actual samples.

关 键 词：毛细管电泳 化妆品 防腐剂 分离测定 

分 类 号：O657.8[理学—分析化学] S482.294[理学—化学]